Mixture of amino acid derivatives, process of producing the mixt

Details Mixture of amino acid derivatives, process of producing the mixt Mixture of amino acid derivatives, process of producing the mixt

1987-02-25

1988-11-15

Richman, Barry S.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436111, 436161, 436172, G01N 2177, G01N 3368

Patent

active

047849622

ABSTRACT:
A mixture of fluorescently-excitable amino acid derivatives suitable for use in quantitative amino acid analysis is provided in which primary amino acids are present as derivatives of a first reagent and secondary amino acids are present as derivatives of a second different reagent. The process for preparing the mixture comprises the steps of derivatization of the primary amino acids of an amino acid sample with ortho-phthalaldehyde (OPA) and of derivatization of the secondary amino acids of the sample with fluorenylmethylchloroformate (FMOC) in the presence of acetonitrile. The mixture can be obtained automatically and is highly suitable for fast analysis by reversed-phase liquid chromatography.

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