Mixture of a non-covalent heterodimer complex and a basic amphip

Details Mixture of a non-covalent heterodimer complex and a basic amphip Mixture of a non-covalent heterodimer complex and a basic amphip

1991-07-23

1993-08-03

Russel, Jeffrey E.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

424 943, 424542, 514 21, 530300, 530324, 530350, 530856, A61K 3702, A61K 3748, C07K 1508

Patent

active

052329119

ABSTRACT:
Novel cytotoxic agent useful against malignant tumors. It provides a stable composition of matter based on the cytotoxic activity of two synergistically acting toxins which sequence are herein described. The basic amphipathic peptide binds to the cell membrane causing perturbation of the lipid bilayer. The non-covalent heterodimer complex dissociates and the phospholiphase A.sub.2 subunit (B) binds to the cell membrane. Subunit A acts as a chaperon preventing non-specific binding of the phospholipase A.sub.2, it has no enzymatic activity. The basic amphipathic peptide increases the effect of the phospholipase A.sub.2 subunit. Cell death is caused by the enzymatic hydrolysis of cell membrance phospholipids. Both the non-covalent heterodimer complex and the basic amphipathic peptide used were purified from the venoms of Crotalus durissus terrificus and Naja naja atra, respectively.

REFERENCES:
patent: 4731439 (1988-03-01), Marquardt et al.
patent: 4774318 (1988-09-01), Marquardt et al.
patent: 5053492 (1991-10-01), Rael et al.
patent: 5164196 (1992-11-01), Plata et al.
Aird et al, "Rattlesnake Presynaptic Neurotoxins . . . ", Biochemistry, vol. 24, 1985, pp. 7054-7058.
Boudnier et al, "Cloning and sequencing of cDNAs encoding the two subunits of Crotoxin", Nucleic Acids Research, 16(18), 1988, p. 9050.
Breithaupt et al, "Isolation and Characterization of Three Phospholipases . . . ", Biochim. Biophys. Acta. vol. 403, 1975, pp. 355-369.
Chemical Abstracts 86:11908d (1977).
Narita et al, "The Amino Acid Sequence of Cardiotoxin From Formosan Cobra . . . ", Biochem. Biophys. Res. Comm. 41(2) 1970, pp. 339-343.
Braganca, Patel & Bardinath, 1967, Biochem, Biophys. Acta 333-345.
Takechi, Sasaki & Hayashi, 1971, Naturwissenshaften, 58, 323.
Cotte, Eddenfeld, Yahr & Calvo Lairet, 1972, Toxicon, 10, pp. 157-163.

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