Mixing and matching TC proteins for pest control

Details Mixing and matching TC proteins for pest control Mixing and matching TC proteins for pest control

2004-01-07

2009-02-17

Prouty, Rebecca E. (Department: 1652)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C424S405000, C530S350000

Reexamination Certificate

active

07491698

ABSTRACT:
The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable fromXenorhabdus, Photorhabdus,andPaenibacillus,can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (fromPhotorhabdus, Xenorhabdus,orPaenibacillus,for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-aloneXenorhabdusprotein toxin as well as a stand-alonePhotorhabdusprotein toxin. (As one skilled in the art knows,Xenorhabdustoxin proteins tend to be more desirable for controlling lepidopterans whilePhotorhabdustoxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.

REFERENCES:
patent: 6048838 (2000-04-01), Ensign et al.
patent: 6174860 (2001-01-01), Kramer et al.
patent: 6277823 (2001-08-01), Kramer et al.
patent: 6281413 (2001-08-01), Kramer et al.
patent: 6590142 (2003-07-01), Petell et al.
patent: 2002/0078478 (2002-06-01), Ffrench-Constant
patent: 2004/0103455 (2004-05-01), Ffrench-Constant et al.
patent: WO 95/00647 (1995-01-01), None
patent: WO 97/17432 (1997-05-01), None
patent: WO 98/08388 (1998-03-01), None
patent: WO 98/08932 (1998-03-01), None
patent: WO 98/50427 (1998-11-01), None
patent: WO 99/03328 (1999-01-01), None
patent: WO 99/42589 (1999-08-01), None
patent: WO 99/54472 (1999-10-01), None
patent: WO 00/30453 (2000-06-01), None
patent: WO 00/42855 (2000-07-01), None
patent: WO 01/11029 (2001-02-01), None
patent: WO 02/094867 (2002-11-01), None
patent: WO 0294867 (2002-11-01), None
patent: WO 2004/002223 (2004-01-01), None
Morgan et al., “Sequence analysis of insecticidal genes fromXenorhabdus nematophilusPMFI296,” Appl Environ Microbiol 67:2062-2069, 2001.
Result 1 from searching SEQ ID No. 34 in the UniProt database on Feb. 17, 2006, alignment with the sequence of Morgan et al. cited in U above.
Result 1 from searching SEQ ID No. 45 in the published patent applications protein database on Feb. 17, 2006, alignment with SEQ ID No. 10 of French-Constant et al. cited in A above.
Result 3 from searching SEQ ID No. 45 in the Geneseq protein database on Feb. 17, 2006, alignment with SEQ ID No. 3319 of Duchaud et al. cited in N above.
Result 1 from searching SEQ ID No. 47 in the published patent applications protein database on Feb. 17, 2006, alignment with SEQ ID No. 12 of Ffrench-Constant et al. cited in A above.
Result 1 from searching SEQ ID No. 47 in the issued patents protein database on Feb. 17, 2006, alignment with SEQ ID No. 12 of Kramer et al. cited in B above.
Alignment of SEQ ID No. 34 and the protein encoded by SEQ ID No. 21, TcdA1 (GenBank record No. AAF05542), from the GenBank Blast web site, http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi, Jun. 21, 2006.
Written Description Guidelines, pp. 35-40, http://www.uspto.gov/web/offices/pac/writtendesc.pdf.
H. Guo et al., “Protein Tolerance to Random Amino Acid Change”, PNAS 101(25):9205-9210, 2004.
Bowen et al., “Insecticidal toxins from the bacteriumPhotorhabdus luminescens,” Science 280 (5372), 2129-2132 (1998).
Bowen et al., Insecticidal toxin complex protein TcaC (Photorhabdus luminescens), Genbank Accession No. AAC38625 (gi:3265037), Jun. 30, 1998.
Bowen et al., Insecticidal toxin complex protein TcbA (Photorhabdus luminescens), Genbank Accession No. AAC38627 (gi:3265040), Jun. 30, 1998.
Ffrench-Constant et al., “Photorhabdustoxins: novel biological insecticides,” Current Opinion in Microbiology (1999), p. 284-288, vol. 12.
Ffrench-Constant et al., “Novel insecticidal toxins from nematode-symbiotic bacteria,” Cellular and Mol. Life Sciences (May. 2000), p. 828-833, vol. 57, No. 5.
Ffrench-Constant et al., “A Genomic Sample Sequence of the Entomopathogenic BacteriumPhotorhabdus. . . ,” Appl. Environ. Microbiol. (Aug. 2000), p. 3310-3329, vol. 66, No. 8.
Forst et al., “Molecular Biology of the Symbiotic-Pathogenic BacteriaXenorhabdusspp. andPhotorhabdusspp.,”Microbiological Reviews (Mar. 1996), p. 21-43, vol. 60, No. 1.
Hurst et al., “Plasmid-Located Pathogenicity Determinants ofSerratia entomophila. . . ,” Journal of Bacteriology (Sep. 2000), p. 5127-5138, vol. 182, No. 18.
Hurst et al., SepA (Serratia entomophila), Genbank Accession No. AAG09642 (gi:9963678) Nov. 5, 2003.
Hurst et al., SepB (Serratia entomophila), Genbank Accession No. AAG09643 (gi:9963679) Nov. 5, 2003.
Hurst et al., SepA (Serratia entomophila), Genbank Accession No. AAG09642 (gi:9963678).
Hurst et al., SepB (Serratia entomophila), Genbank Accession No. AAG09643 (gi:9963679).
Hurst et al., SepC (Serratia entomophila), Genbank Accession No. AAG09644 (gi:9963980) Nov. 5, 2003.
Merlo et al., Toxin A (Photorhabdus luminescens), Genbank Accession No. AAF05542 (gi:6176340), Nov. 2, 1999.
Morgan et al., “Sequence Analysis of Insecticidal Genes fromXenorhabdus nematophilusPMFI296,” Appl. Environ. Microbiol. (May 2001), p. 2062-2069, vol. 67, No. 5.
Morgan et al.,Xenorhabdus nematophilusgenes, Genbank Accession No. AJ308438, May 11, 2001.
Morgan et al., Putative chitinase (Xenorhabdus nematophila), Genbank Accession No. CAC38398 (gi:14041727), May 11, 2001.
Morgan et al., XptA1 protein (Xenorhabdus nematophila), Genbank Accession No. CAC38401 (gi:14041730), May 11, 2001.
Morgan et al., XptB1 protein (Xenorhabdus nematophila), Genbank Accession No. CAC38402 (gi:14041731), May 11, 2001.
Morgan et al., XptC1 protein (Xenorhabdus nematophila), Genbank Accession No. CAC38403 (gi:14041732), May 11, 2001.
Morgan et al., XptA2 protein (Xenorhabdus nematophila), Genbank Accession No. CAC38404 (gi:14041733), May 11, 2001.
NCBI Microbial Genomes Annotation Project, COG3209: Rhs family protein (Pseudomonas. . . ), Genbank Accession No. ZP—00083266 (gi:23058175) Apr. 9, 2004.
Hurst et al., SepC (Serratia entomophila), Genbank Accession No. AAG09644 (gi:9963980).
NCBI Microbial Genomes Annotation Project, Hypothetical protein (Pseudomonas fluorescens. . . ), Genbank Accession No. ZP—00084399 (gi:23059431) Apr. 9, 2004.
NCBI Microbial Genomes Annotation Project, COG3209: Rhs family protein (Pseudomonas. . . ), Genbank Accession No. ZP—00084400 (gi:23059432) Apr. 9, 2004.
NCBI Microbial Genomes Annotation Project, COG3209: Rhs family protein (Pseudomonas. . . ), Genbank Accession No. ZP—00084401 (gi:23059433) Apr. 9, 2004.
NCBI Microbial Genomes Annotation Project, COG3209: Rhs family protein (Pseudomonas. . . ), Genbank Accession No. ZP—00084403 (gi:23059435) Apr. 9, 2004.
NCBI Microbial Genomes Annotation Project, COG3209: Rhs family protein (Pseudomonas. . . ), Genbank Accession No. ZP—00125560 (gi:23470227) Sep. 23, 2003.
NCBI Microbial Genomes Annotation Project, Hypothetical protein (Pseudomonas syringae. . . ), Genbank Accession No. ZP—00126265 (gi:23470933) Sep. 23, 2003.
NCBI Microbial Genomes Annotation Project, COG3209: Rhs family protein (Pseudomonas. . . ), Genbank Accession No. ZP—00127864 (gi:23472540) Apr. 9, 2004.
NCBI Microbial Genomes Annotation Project, COG3209: Rhs family protein (Pseudomonas. . . ), Genbank Accession No. ZP—00127865 (gi:23472541) Ap

Affiliated with

Also associated with

Rating

Mixing and matching TC proteins for pest control does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mixing and matching TC proteins for pest control, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mixing and matching TC proteins for pest control will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4083840