Mitochondrial P450

Details Mitochondrial P450 Mitochondrial P450

1996-08-26

1997-09-16

Vogel, Nancy T.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

43525121, 4353201, 435 691, C12N 902, C12N 1581, C12N 119, C12N 1500

Patent

active

056680007

ABSTRACT:
The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome.
An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5.beta.-cholestane-3.alpha., 7.alpha., 12.alpha., 27-tetrol, 25-hydroxyvitamin D.sub.3 and 1.alpha.,25-dihydroxyvitamin D.sub.3 are also disclosed.

REFERENCES:
patent: 4766068 (1988-08-01), Oeda et al.
Hurt et al., "The Cleavable Prepiece of COXIV carries all the information for Importing an Attached Cytosolic Protein into the Yeast Mitochondrial Matrix," In: Protein Transport and Secretion, Gething, M.J. (ed.) CSHL, Cold Spring Harbor, NY. (1984).
Suet al, "A cDNA encoding a Rat Mitochondrial Cytochrome P450 Catalyzing Both the 26-Hydroxylation of Cholesterol and 25-Hydroxylation of Vitamin D.sub.3 : Gonadotropic Regulation of the Cognate mRNA in Ovaries,"DNA and Cell Biology 9(9):657-665. (Sep. 1990).
Bjorkhem et al, "Properties of a Reconstituted Vitamin D.sub.3 25-Hydroxylase from Rat Liver Mitochondria," JBC 255(11):5244-49. (1980).
Zuber et al., "Simultaneous transfection of COS-1 cells with mitochondrial and microsomal steroid hydroxylases:Incorporation of a steroidogenic pathway into nonsteroidogenic Celk," PNAS, 85:699-703 (Feb. 1988).
Kingsman et al., "Heterologous Gene Expression in Saccharomyces Cerevisiae," Biotechnology and Bioengineering Reviews, 3:377-415 (1985).
Barnes et al., Proc. Natl. Acad. Sci. USA 88, 5597-5601 (1991).
Zuber et al., Science, 234, 1258-1261 (1986).
Usui et al., Febs Letters, 262(1) 135-138 (1990).
Usui et al., Febs Letters, 274(1,2) 175-177 (1990).
Oeda et al., DNA 4, 203-210 (1985).
Sakaki et al., DNA 8, 409-418 (1989).
Shibata et al., DNA Cell Biol. 9, 27-36 (1990).
Akiyoshi-Shibata et al., Febs Letters 280, 367-370 (1991).
Chemical Abstracts 113:2514w.
Chemical Abstracts 108:218092b.
Okuda et al., J. Biol. Chem., 263, 18138-18142 (1988).
Masumoto et al., J. Biol. Chem., 263, 14256-14260 (1988).
Patent Abstracts Of Japan, vol. 12, No. 110 (C486) (2957) Apr. 8, 1988, JP 62-236495.
Emiko Usui, et al., Molecular cloning of cDNA for vitamin D.sub.3 25-hydroxylase from rat liver mitochondria, FEBS, Mar. 1990, vol. 262(1), pp. 135-138.
Biochemical Dictionary; p. 41, c. 1994 by Tokyo Kagaku Dojinkai.

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