Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Animal cell – per se – expressing immunoglobulin – antibody – or...
Reexamination Certificate
2006-12-12
2006-12-12
Haddad, Maher M. (Department: 1644)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Animal cell, per se, expressing immunoglobulin, antibody, or...
C435S975000, C530S388260
Reexamination Certificate
active
07148063
ABSTRACT:
The object of the present invention is to provide a method for more accurately assaying the enzyme activities of mCK isozymes and a method for separately assaying the enzyme activities of CK isozymes by separately assaying the enzyme activities of ubiquitous mCK (umCK) and sarcomeric mCK (smCK). The above object is attained by an assay using an antibody that specifically recognizes umCK protein. In addition, other anti-mCK antibodies (e.g., anti-smCK antibody) and/or anti-human CK-M-inhibiting antibody can also be used. The above antibody is a polyclonal antibody or a monoclonal antibody. As a result of these antibodies, a monoclonal antibody (U1-1881) that is capable of specifically recognizing human umCK and is produced by a hybridoma having a deposition number of FERM BP-8342 is provided.
REFERENCES:
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patent: 6586194 (2003-07-01), Shirahase et al.
Thomure et al. Regulation of creatine kinase isoenzymes in human placenta during early, mid-, and late gestation. J Soc Gynecol Investig. Nov.-Dec. 1996;3(6):322-7.
Schlattner et al. Divergent enzyme kinetics and structural properties of the two human mitochondrial creatine kinase isoenzymes. Biol Chem. Nov. 2000;381(11):1063-70.
Harlow E, Lane D.. Antibodies a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press,pp. 141-149 and 151-155, 1989.
Kajita Tadahiro
Kishi Koji
Shirahase Yasushi
Yamashita Kazuaki
Haddad Maher M.
Sughrue & Mion, PLLC
Sysmex Corporation
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