Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Patent
1999-04-02
2000-10-24
Therkorn, Ernest G.
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
210656, 210659, 2101982, B01D 1508
Patent
active
06136195&
ABSTRACT:
An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation solution supply means, and a separation solution conduit communicating with the separation column and the separation solution supply means, and a cleaning solution valve means positioned in the separation solution conduit for injecting cleaning solution into the separation solution conduit. A process for cleaning the non-polar DNA separation surfaces in the apparatus comprising interrupting the flow of separation solvent with a block of cleaning solution injected into the flow of separation solution passing to the column, the cleaning solution containing agent which removes accumulated residues from the non-polar surface. The cleaning solution can have an alkaline pH and contain a chelating agent such as EDTA.
REFERENCES:
patent: 5100547 (1992-03-01), Hardiman et al.
patent: 5585236 (1996-12-01), Bonn et al.
patent: 5651931 (1997-07-01), Bailey et al.
patent: 5772889 (1998-06-01), Gjerde
patent: 5795976 (1998-08-01), Oefner et al.
patent: 5972222 (1999-10-01), Gjerde
patent: 5997742 (1999-12-01), Gjerde
patent: 6056877 (2000-05-01), Gjerde
All-Chrom Newsletter Metal Components, a Potential Source of Interference in HPLC Analysis, Alltech-Applied Science vol. 25, No. 1, Jun., 1986 pp. 1-6.
Apffel et al. Applications of HPLC for the Analysis of Doublse Stranded DNA use of Wide Pore Sisilca Based Materials, ISPPP '97 17th International Symposium on the Separation of Proteins, Peptides & Polynucleotides, Oct. 26-29, 1997 pp. 1-5.
DHPLC Workshop, Stanford University, CA, pp. 32-43 (Mar. 17, 1997).
Abstract of Taylor et al, Strand Specificity of Nicking of DNA and Chi Sites by RECBCD Enzyme, JBC Online, vol. 270 No. 41: 24459-24467 (Oct. 1995).
Huang et al. Large-Scale Purification of Synthetic Oligonucleotides and Carcinogen-Modified Oligodeoxynucleotides on a Revers-Phase Polystyrene (PRP-1) Column, Analytical Biochemistry, 190, 21-25, 1990.
Huber et al, High-Respolution Liquid Chromatography of DNA Fragments on Non-Porous Poly9styrene-Divinylbenzene) Particles, Nucleic Acid Research, vol. 21 No. 5: 1061-1066 (1993).
Wells et al. RPC-5 Column Chromatography for the Isolation of DNA Fragments, Methods of Enzymology, vol. 65, Nucleic Acids, Part I, pp. 327-347, 1980.
Gjerde Douglas T.
Haefele Robert M.
Lamb Kimberly A.
Taylor Paul D.
Therkorn Ernest G.
Transgenomic Inc.
Walker William B.
LandOfFree
MIPC column cleaning system and process does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with MIPC column cleaning system and process, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and MIPC column cleaning system and process will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1960055