Minimum procedure system for the determination of analytes

Details Minimum procedure system for the determination of analytes Minimum procedure system for the determination of analytes

1986-08-13

1990-06-19

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 25, 435 28, 435805, 435808, 435810, 436 24, 436 46, 436 63, 436 95, 436166, 436169, 422 56, 422 57, 422 58, 422 61, 422 681, C12Q 154, C12Q 132

Patent

active

049353462

ABSTRACT:
A method for determining the presence of an analyte in a fluid is described along with various components of an apparatus specifically designed to carry out the method. The method involves taking a reflectance reading from one surface of an inert porous matrix impregnated with a reagent that will interact with the analyte to produce a light-absorbing reaction product when the fluid being analyzed is applied to another surface and migrates through the matrix to the surface being read. Reflectance measurements are made at two separate wavelengths in order to eliminate interferences, and a timing circuit is triggered by an initial decrease in reflectance by the wetting of the surface whose reflectance is being measured by the fluid which passes through the inert matrix. The method and apparatus are particularly suitable for the measurement of glucose levels in blood without requiring separation of red blood cells from serum or plasma.

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