Optics: measuring and testing – By shade or color – With color transmitting filter
Reexamination Certificate
2007-06-28
2010-11-23
Geisel, Kara E (Department: 2877)
Optics: measuring and testing
By shade or color
With color transmitting filter
Reexamination Certificate
active
07839507
ABSTRACT:
Methods and systems for processing signals to minimize the effects of dye crosstalk. Deconvolution of multiplexed dye signals can include corrections for residual error determined from experimental measurements. Residual error corrections can account for reaction or assay specific factors and modify the subsequent filtering of signals. Correction values can be determined for specific dye-probe conjugates to minimize dye crosstalk and may be combined with residual error correction to further minimize errors in spectral deconvolution. Apparatus, systems, and computer-readable media can process signals and modify filters based on values obtained using the methods.
REFERENCES:
patent: 6333501 (2001-12-01), LaBrenz
patent: 6863791 (2005-03-01), Liu et al.
patent: 7035739 (2006-04-01), Schadt et al.
patent: 7209836 (2007-04-01), Schermer et al.
patent: 2004/0259260 (2004-12-01), Gunstream et al.
patent: 2007/0100569 (2007-05-01), DeSimas et al.
Yin et al. “Automatic matrix determination in four dye fluorescence-based DNA sequencing”, 1996, Eletrophoresis, vol. 17, pp. 1143-1150.
Lewin et al., Quantiative DNA Methylation Anaylsis Based on Four-Dye Trace Data from Direct Sequencing of PCR Amplificates, Bioinformatics, vol. 20, pp. 3005-3012, 2004.
International Search Report for Int'l application No. PCT/US07/72383 dated Jul. 29, 2008, along with Written Opinion of the International Searching Authority.
Bodeau John P.
Gunstream Stephen J.
McCoy Mark A.
Woo David C.
Applied Biosystems LLC
Geisel Kara E
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