Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Chemical stimulation of growth or activity by addition of...
Patent
1986-07-14
1992-01-14
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Chemical stimulation of growth or activity by addition of...
435 34, 435254, 435922, 435924, C12N 138, C12N 114, C12Q 104
Patent
active
050810332
ABSTRACT:
Methods and compositions are disclosed for the rapid identification of fungal pathogens. In a preferred method, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive medium, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter plating a sample of said visible fungi on miniature culture plates for example having the dimensions of about 33.times.75.times.5 millimeters, said plates containing differential fungal media, and thereafter identifying funal pathogens in a manner consistent with said medium selected.
In a related method utilizing the differential carbohydrate assimilation properties of fungal pathogens, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive media, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter inoculating a carbohydrate depleted medium with a sample of said visible fungi, incubating said inoculum for a period of time, thereafter plating a sample of said inoculated carbohydrate depleted medium on miniature culture plates, said plates containing differential carbohydrate assimilation media, and thereafter identifying said fungal pathogens in a manner consistent with said medium selected.
Novel compositions useful in practicing the disclosed methods are provided. These include an improved fungal growth medium, the improvement comprising the novel addition of ammonium ions to a medium comprising purified saponin, oxgall, a substrate for phenol oxidase and a supporting agent.
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Dorn Gordon L.
Fleming William H.
Knezek Karen L.
Robinson Douglas W.
Wadley Technologies, Inc.
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