Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1986-07-14
1989-07-11
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435255, 435922, C12Q 104, C12N 116
Patent
active
048471283
ABSTRACT:
Methods and compositions are disclosed for the rapid identification of fungal pathogens. In a preferred method, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive medium, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter plating a sample of said visible fungi on miniature culture plates for example having the dimensions of about 33.times.75.times.5 millimeters, said plates containing differential fungal media, and thereafter identifying fungal pathogens in a manner consistent with said medium selected.
In a related method utilizing the differential carbohydrate assimilation properties of fungal pathogens, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive media, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter inoculating a carbohydrate depleted medium with a sample of said visible fungi, incubating said inoculum for a period of time, thereafter plating a sample of said inoculated carbohydrate depleted medium on miniature culture plates, said plates containing differential carbohydrate assimilation media, and thereafter identifying said fungal pathogens in a manner consistent with said medium selected.
Novel compositions useful in practicing the disclosed methods are provided. These include an improved fungal growth medium, the improvement comprising the novel addition of ammonium ions to a medium comprising purified saponin, oxgall, a substrate for phenol oxidase and a supporting agent.
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Scherr
Dorn Gordon L.
Fleming William H.
Knezek Karen L.
Deck Randall E.
Wadley Technologies, Inc.
Warden Robert J.
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