Miniaturized yeast identification system

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435255, 435922, C12Q 104, C12N 116

Patent

active

048471283

ABSTRACT:
Methods and compositions are disclosed for the rapid identification of fungal pathogens. In a preferred method, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive medium, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter plating a sample of said visible fungi on miniature culture plates for example having the dimensions of about 33.times.75.times.5 millimeters, said plates containing differential fungal media, and thereafter identifying fungal pathogens in a manner consistent with said medium selected.
In a related method utilizing the differential carbohydrate assimilation properties of fungal pathogens, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive media, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter inoculating a carbohydrate depleted medium with a sample of said visible fungi, incubating said inoculum for a period of time, thereafter plating a sample of said inoculated carbohydrate depleted medium on miniature culture plates, said plates containing differential carbohydrate assimilation media, and thereafter identifying said fungal pathogens in a manner consistent with said medium selected.
Novel compositions useful in practicing the disclosed methods are provided. These include an improved fungal growth medium, the improvement comprising the novel addition of ammonium ions to a medium comprising purified saponin, oxgall, a substrate for phenol oxidase and a supporting agent.

REFERENCES:
patent: 2561339 (1951-07-01), Chediak
patent: 3073750 (1963-01-01), Greenblatt
patent: 3097070 (1963-07-01), Aldrich
patent: 3649463 (1972-03-01), Buterbaugh
patent: 3728228 (1973-04-01), Duranty
patent: 3870600 (1975-03-01), Youssef
patent: 3875015 (1975-04-01), Wadley et al.
patent: 3883398 (1975-05-01), Ono
patent: 3883425 (1975-05-01), Dorn
patent: 3928136 (1975-12-01), Launey
patent: 4030980 (1977-06-01), Beckford et al.
patent: 4144133 (1979-03-01), Dorn et al.
patent: 4179266 (1979-12-01), Lukacsek
Difco Manual, 9th Ed., 1977, Difcon Laboratories Chic., Detroit, MI, 48201, pp. 294-295.
Mardon, et al; "Control of Dimorphism in Biochemical Variant of Candida albicans", 72 Chemical Abstracts 96 (1970) (No. 19405u).
Land, G. A. et al; "Factors Affecting Filamentation in Candida albicans Relation of the Uptake and Distribution of Proline to Morphogenesis", 83 Chemical Abstracts (1975) (No. 144337b).
Manning, et al; "Strain Variation and Morphogenesis of Yeast-phase and Mycelial-phase Candida albicans in Low Sulfate Synthetic Medium", 70 Biological Abstracts (No. 67493) (1977).
Fleming, et al., "New Culture Medium for the Presumptive Identification of Candida albicans and Cryptococcus neoformans", 64 Biological Abstracts #3119 (1977).
Fleming, et al., "New Culture Medium for the Presumptive Identification of Candida albicans and Cryptococcus neoformans", 5 J. Clinical Microbiology 236-343 (1977).
Hrmova et al, "Induction of Mycelial Type of Development in Candida albicans by Low Glucose Concentration", 76 Mycopathologia 83-96 (1981).
Casal et al, "The Comparison of Six Media for Chlamydospore Production by Candida albicans", 76 Mycopathologia, 125-128 (1981).
Hedden et al, "A Reemphasis-Germ Tubes Diagnostic for Candida albicans Having no Constrictions", 70 Mycopathologia 95-101 (1980).
Gunasekaran et al, "A Simple Liquid Medium for Chlamydospore Formation in Candida albicans", 64 Mycopathologia, 143-146 (1978).
Gunasekaran et al, "A Simple Medium for Isolation and Identification of Candida albicans, Directly from Clinical Specimens", 61 Mycopathologia 151-157 (1977).
Warwood et al, "Comparison of Cream of Rice Agar and Horse Serum for Differentiating Germ Tubes of Candida albicans from Filaments of Candida tropicalis", 5 J. Clinical Microbiology 501-502 (1977).
Auger et al, "Factors Influencing Germ Tube Production in Candida albicans", 61 Mycopathologia 183-186 (1977).
Odds et al, "Routine Use of Modified Sabouraud's Dextrose Broth in the Germ Tube Test for Candida albicans", 32 Medical Laboratory Technology 23-26 (1975).
Lee et al, "An Amino Acid Liquid Synthetic Medium for the Development of Mycelial and Yeast Forms of Candida albicans", 13 Sabouraudia, 148-153 (1975).
Beheshti, "Germ Tube and Chlamydospore Formation by Candida albicans on a New Medium", 2 J. Clinical Microbiology 345-348 (1975).
Land et al, "Improved Auxanographic Method for Yeast Assimilations: A Comparison with Other Approaches", 2 J. Clinical Microbiology 206-217 (1975).
Land et al, "Factors Affecting Filamentation in Candida albicans: Changes in Respiratory Activity of Candida albicans During Filamentation", 12 Infection and Immunity 119-127 (1975).
Land et al, "Factors Affecting Filamentation in Candida albicans: Relationship of the Uptake and Distribution of Proline to Morphogenesis", 11 Infection and Immunity 1014-1023 (1975).
Evans et al, "Optimum Conditions for Initiation of Filamentation in Candida albicans", 21 Canadian J. Microbiology, 338-342 (1975).
Nishioka et al, "Dimorphism, Sensitivity to Nystatin and Acriflavin Uptake in a Strain of Candida albicans grown with Glutamate as a Sole Nitrogen and Carbon Source", 12 Sabouraudia 295-301 (1974).
Joshi et al, "The Formation of Germ Tubes by Candida albicans in Various Peptone Media", 11 Sabouraudia 259-262 (1973).
Joshi et al, "The Formation of Germ Tubes by Candida albicans in Sheep Serum and Trypticase Soya Broth", 60 Am. J. Clinical Pathology 839-842 (1973).
Chattaway et al, "An Examination of the Production of Hydrolytic Enzymes and Toxins by Pathogenic Strains of Candida albicans", 67 J. General Microbiology 255-263 (1971).
Dolan, "A Practical Approach to Identification of Yeast-Like Organisms", 55 Am. J. Clinical Pathology 580-590 (1971).
Dolan et al, "Further Studies of the Germ Tube Test for Candida albicans Identification", 55 Am. J. Clinical Pathology, 733-734 (1971).
Jansons et al, "Induction, Morphogenesis and Germination of the Chlamydospore of Candida albicans", 104 J. Bacteriology, 910-921 (1970).
Mardon et al, "Control of Dimorphism in a Biochemical Variant of Candida albicans", 100 J. Bacteriology, 701-707 (1969).
Bernander et al, "Growth and Phase Conversion of Candida albicans in Dubos Medium", 7 Sabouraudia 146-155 (1969).
Chattaway et al, "Cell Wall Composition of the Mycelial and Blastospore forms of Candida albicans", 51 J. General Microbiology 367-376 (1968).
Landau et al, "The Rapid Formation in Serum of Filaments by Candida albicans", 44 J. Investigative Dermatology 171-179 (1965).
Mackenzie, "Studies on the Morphogenesis of Candida albicans II. Growth in Organ Extract", 4 Sabouraudia, 126-130 (1965).
Widra, "Phosphate Directed Y-N Variation in Candida albicans", 23 Mycopathologia, 197-202 (1964).
Buckley et al, "The Identification of Candida albicans Within Two Hours by the Use of an Egg White Slide Preparation", 2 Sabouraudia 205-208 (1963).
Duncan et al, "A Comparison of Media for the Production of Chlamydospores by Candida albicans", 29 Am. J. of Medical Technology, 199-206 (Jul.-Aug. 1963).
Mackenzie, "Serum Tube Identification of Candida albicans", 15 Journal of Clinical Pathology 563-565 (1962).
Bakerspigel, "Sodium Taurocholate Medium for the Identification of Candida albicans", 83 Journal of Bacteriology 694 (1962).
Newton, et al, "Further Work on the Rapid Identification of Candida albicans", 35 Journal of Investigative Dermatology 123 (1960).
Taschdjian, et al, "Rapid Identification of Candida albicans by Filamentation on Serum and Serum Substitutes", 99 Journal of Diseases of Children 212-215 (1960).
Kelly et al, "Candida albicans: A Study of Media Designed to Promote Chlamydospore Production", 53 Journal of Laboratory Clinical Medicine, 807-809 (1959).
Nickerson, "Roll of Nutrition in the Maintenance of the Yeast Shape in Candida", 40 Am. Journal of Botany 584-592 (1953).
Johnson, "Candida (Monilia) albicans", A.M.A. Archives of Dermatalogy & Syphilogy 49-59 (1953).
Scherr

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Miniaturized yeast identification system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Miniaturized yeast identification system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Miniaturized yeast identification system will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-436453

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.