Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Utility Patent
1998-01-12
2001-01-02
Beisner, William H. (Department: 1744)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C435S006120, C435S287900, C435S288600
Utility Patent
active
06168948
ABSTRACT:
BACKGROUND OF THE INVENTION
The relationship between structure and function of macromolecules is of fundamental importance in the understanding of biological systems. These relationships are important to understanding, for example, the functions of enzymes, structure of signaling proteins, ways in which cells communicate with each other, as well as mechanisms of cellular control and metabolic feedback.
Genetic information is critical in continuation of life processes. Life is substantially informationally based and its genetic content controls the growth and reproduction of the organism. The amino acid sequences of polypeptides, which are critical features of all living systems, are encoded by the genetic material of the cell. Further, the properties of these polypeptides, e.g., as enzymes, functional proteins, and structural proteins, are determined by the sequence of amino acids which make them up. As structure and function are integrally related, many biological functions may be explained by elucidating the underlying structural features which provide those functions, and these structures are determined by the underlying genetic information in the form of polynucleotide sequences. In addition to encoding polypeptides, polynucleotide sequences can also be specifically involved in, for example, the control and regulation of gene expression.
The study of this genetic information has proved to be of great value in providing a better understanding of life processes, as well as diagnosing and treating a large number of disorders. In particular, disorders which are caused by mutations, deletions or repeats in specific portions of the genome, may be readily diagnosed and/or treated using genetic techniques. Similarly, disorders caused by external agents may be diagnosed by detecting the presence of genetic material which is unique to the external agent, e.g., bacterial or viral DNA.
While current genetic methods are generally capable of identifying these genetic sequences, such methods generally rely on a multiplicity of distinct processes to elucidate the nucleic acid sequences, with each process introducing a potential for error into the overall process. These processes also draw from a large number of distinct disciplines, including chemistry, molecular biology, medicine and others. It would therefore be desirable to integrate the various process used in genetic diagnosis, in a single process, at a minimum cost, and with a maximum ease of operation.
Interest has been growing in the fabrication of microfluidic devices. Typically, advances in the semiconductor manufacturing arts have been translated to the fabrication of micromechanical structures, e.g., micropumps, microvalves, and the like, and microfluidic devices including miniature chambers and flow passages.
A number of researchers have attempted to employ these microfabrication techniques in the miniaturization of some of the processes involved in genetic analysis in particular. For example, published PCT Application No. WO 94/05414, to Northrup and White, incorporated herein by reference in its entirety for all purposes, reports an integrated micro-PCR apparatus for collection and amplification of nucleic acids from a specimen. However, there remains a need for an apparatus which combines the various processing and analytical operations involved in nucleic acid analysis. The present invention meets these and other needs.
SUMMARY OF THE INVENTION
The present invention generally provides miniature integrated fluidic systems for carrying out a variety of preparative and analytical operations, as well as methods of operating these systems and methods of using these systems.
REFERENCES:
patent: 4426451 (1984-01-01), Columbus
patent: 4490216 (1984-12-01), McConnell
patent: 4591550 (1986-05-01), Hafeman et al.
patent: 4676274 (1987-06-01), Brown
patent: 4704353 (1987-11-01), Humphries et al.
patent: 4758786 (1988-07-01), Hafeman
patent: 4789628 (1988-12-01), Nayak
patent: 4790640 (1988-12-01), Nason
patent: 4849330 (1989-07-01), Humphries et al.
patent: 4883579 (1989-11-01), Humphries et al.
patent: 4911794 (1990-03-01), Parce et al.
patent: 4915812 (1990-04-01), Parce et al.
patent: 4963815 (1990-10-01), Hafeman
patent: 5126022 (1992-06-01), Soane et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5164319 (1992-11-01), Hafeman et al.
patent: 5171132 (1992-12-01), Miyazaki et al.
patent: 5188963 (1993-02-01), Stapleton
patent: 5229297 (1993-07-01), Schnipelsky et al.
patent: 5230866 (1993-07-01), Shartle et al.
patent: 5252294 (1993-10-01), Kroy et al.
patent: 5271724 (1993-12-01), van Lintel
patent: 5277556 (1994-01-01), van Lintel
patent: 5281516 (1994-01-01), Stapleton et al.
patent: 5296375 (1994-03-01), Kricka et al.
patent: 5304487 (1994-04-01), Wilding et al.
patent: 5346672 (1994-09-01), Stapleton et al.
patent: 5375979 (1994-12-01), Trah
patent: 5382511 (1995-01-01), Stapleton
patent: 5384261 (1995-01-01), Winkler et al.
patent: 5395503 (1995-03-01), Parce et al.
patent: 5424186 (1995-06-01), Fodor et al.
patent: 5436129 (1995-07-01), Stapleton
patent: 5451500 (1995-09-01), Stapleton
patent: 5486335 (1996-01-01), Wilding et al.
patent: 5498392 (1996-03-01), Wilding et al.
patent: 5500188 (1996-03-01), Hafeman et al.
patent: 5580523 (1996-12-01), Bard
patent: 5587128 (1996-12-01), Wilding et al.
patent: 5589350 (1996-12-01), Bochner
patent: 5653939 (1997-08-01), Hollis et al.
patent: 5660993 (1997-08-01), Cathey et al.
patent: 5726026 (1998-03-01), Wilding et al.
patent: 5843767 (1998-12-01), Beattie
patent: 5858195 (1999-01-01), Ramsey
patent: 5863801 (1999-01-01), Southgate
patent: 5876918 (1999-03-01), Wainwright et al.
patent: 5952173 (1999-09-01), Hansmann et al.
patent: 5976336 (1999-11-01), Dubrow et al.
patent: 6001229 (1999-12-01), Ramsey
patent: 6001231 (1999-12-01), Kopf-Sill
patent: 6010607 (2000-01-01), Ramsey
patent: 6010608 (2000-01-01), Ramsey
patent: 6033546 (2000-03-01), Ramsey
patent: WO 90/04645 (1990-05-01), None
patent: WO 90/15070 (1990-12-01), None
patent: WO 92/10092 (1992-06-01), None
patent: WO 93/09668 (1993-05-01), None
patent: WO 94/03791 (1994-02-01), None
patent: WO 94/05414 (1994-03-01), None
patent: WO 98/52691 (1998-11-01), None
Anderson et al., “Miniaturized genetic-analysis system,”Technical Digest of 1996 Solid-State Sensor and Actuator Workshop, Hilton Head Island, South Carolina, pp. 258-261 (1996).
Anderson et al., “Microfluidic biochemical analysis system,”Technical Digest of Transducers '97, International Conference on Solid-State Sensors and Actuators. Chicago, p. 477-480 (1997).
Anderson et al., “Microfluidic Genetic Analysis Systems: Improvements and Methods,”Solid-State Sensor and Actuator Workshop, (Jun. 7-11, 1998) 4 pages total.
Anderson et al., “Microfluidic Genetic Analysis Systems: Improvements and Methods,”Abstract for 1998 Solid-State Sensor and Actuator Workshop, (Jun. 7-11, 1998) 4 pages total.
Andersson et al.,Technical Digest of Transducers '97, International Conference on Solid-State Sensors and Actuators, Chicago, p. 1311-1314 (1997).
Bart et al., “Microfabricated Electrohydrodynamic Pumps,”Sensors and Actuators, A21-A23:193-197 (1990).
Bousse et al., “Biosensors for Detection of Enzymes Immobilized in Microvolume Reaction Chambers,”Sensors and Actuators, B1:555-560 (1990).
Effenhauser et al., “Glass Chips for High-Speed Capillary Electrophoresis Separations with Submicrometer Plate Heights,”Anal. Chem., 65:2637-2642 (1993).
Effenhauser et al., “High-Speed Separation of Antisense Oligonucelotides on a Micromachined Capillary Electrophoresis Device,”Anal. Chem., 66:2949-2953 (1994).
Fodor et al., “Light-Directed, Spatially Addressable Parallel Chemical Synthesis,”Science, 251:767-777 (1991).
Ghandi, “Lithographic processes,”VLSI Fabrication Principles, 2nd ed., John Wiley & Sons, Inc., Ch. 10, pp. 662-703 (1994).
Harrison et al., “Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip,”Science, 261:895-897 (1993).
Harrison et al., “Immunoassay Systems on Chip,”Technical Digest of 1996 Solid-State Sen
Anderson Rolfe C.
Fodor Stephen P. A.
Lipshutz Robert J.
Rava Richard P.
Affymetrix Inc.
Beisner William H.
Townsend and Townsend / and Crew LLP
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