Mini Mu containing plasmid and a method for rapid DNA sequencing

Details Mini Mu containing plasmid and a method for rapid DNA sequencing Mini Mu containing plasmid and a method for rapid DNA sequencing

1984-12-13

1987-12-29

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 4351723, 435320, C12Q 170, C12Q 168, C12N 1500

Patent

active

047161051

ABSTRACT:
The present invention discloses a rapid method of sequencing a relatively large segment of deoxyribonucleic acid. The method in part comprises high frequency insertion of a suitable transposon into a segment of DNA of interest. Preferable use of Mu transposons is described. A plasmid having mini-Mu transposons has been prepared and disclosed.

REFERENCES:
Ray et al., Gene, 4:109-119, 1978.
Gijsegem et al., Plasmid 7:30-44, 1982.
Mizuuchi, et al., "The Extent of DNA Sequence Required for a Functional Bacterial Attachment Site of Phage Lambda", Nucleic Acids Research, 13:1193-1208 (1985).
Craigie, et al., Cloning of the A Gene of Bacteriophage Mu and Purification of Its Product, the Mu Transposase, J. of Biol. Chem. 260:1832-35 (1985).
Mizuuchi, In Vitro Transposition of Bacteriophage Mu: A Biochemical Approach to a Novel Replication Reaction, Cell. 35:785-94 (12/83).
Maxam et al., A New Method for Sequencing DNA. Proc. Natl. Acad. Sci., 74:560-64 (1977).
Sanger et al., Cloning in Single-Stranded Bacteriophage as an Aid to Rapid DNA Sequencing. J. Mol. Biol., 143:161-178 (1980).
Biggin, et al., Buffer Gradient Gels and .sup.35 S Label as an Aid to Rapid DNA Sequence Determination. Proc. Natl. Acad. Sci., 80:3963-65 (1983).
Sanger, et al., DNA Sequencing with Chain-Terminating Inhibitors., 74:5463-67, (1977).

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