Microwell biochip

Details Microwell biochip Microwell biochip

2007-05-15

2007-05-15

Forman, BJ (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S174000, C435S283100, C435S287200, C435S288400, C422S068100, C536S023100, C530S300000

Reexamination Certificate

active

10823021

ABSTRACT:
Microwell biochips (11) are formed from a thin flat plate (13) of polymeric material having a plurality of regularly spaced holes (15) that extend completely therethrough and create microwells. The lower end of each hole is closed by a microporous, hydrophobic, polymeric membrane (17) laminated to the undersurface of the plate which retains an aqueous test solution in the wells until a vacuum is applied to the undersurface thereof to effect draining of the solution and of any wash solution that might be subsequently added. A spot of polymerizing isocyanate-functional hydrogel is applied generally centrally to the porous membrane surface at the bottom of each well in a manner so as to cover only a minor portion of the surface and out of contact with the well sidewalls, thus leaving substantial surface area through which drainage can be readily effected. Biological capture agents are associated with the polymerizing hydrogel so as to become immobilized as a part thereof.

REFERENCES:
patent: 3939105 (1976-02-01), Jones et al.
patent: 4493815 (1985-01-01), Fernwood et al.
patent: 4526690 (1985-07-01), Kiovsky et al.
patent: 4734192 (1988-03-01), Champion et al.
patent: 4777021 (1988-10-01), Wertz et al.
patent: 4797259 (1989-01-01), Matkovich et al.
patent: 4921809 (1990-05-01), Schiff et al.
patent: 5009780 (1991-04-01), Sarrasin
patent: 5047215 (1991-09-01), Manns
patent: 5116496 (1992-05-01), Scott
patent: 5141719 (1992-08-01), Fernwood et al.
patent: 5264184 (1993-11-01), Aysta et al.
patent: 5326533 (1994-07-01), Lee et al.
patent: 5554536 (1996-09-01), Rising
patent: 5667976 (1997-09-01), Van Ness et al.
patent: 5674395 (1997-10-01), Stankowski et al.
patent: 5679310 (1997-10-01), Manns
patent: 5843767 (1998-12-01), Beattie
patent: 6027695 (2000-02-01), Oldenburg et al.
patent: 6083761 (2000-07-01), Kedar et al.
patent: 6146854 (2000-11-01), Koster et al.
patent: 6159368 (2000-12-01), Moring et al.
patent: 6200533 (2001-03-01), Blevins et al.
patent: 6235520 (2001-05-01), Malin et al.
patent: 6372813 (2002-04-01), Johnson et al.
patent: 0339769 (1989-11-01), None
patent: 2176601 (1986-12-01), None
patent: WO 98/14277 (1998-04-01), None
patent: WO 00/50644 (2000-08-01), None

Affiliated with

Also associated with

Rating

Microwell biochip does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Microwell biochip, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microwell biochip will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3742997