Microwave acceleration of enzyme-catalyzed modification of macro

Details Microwave acceleration of enzyme-catalyzed modification of macro Microwave acceleration of enzyme-catalyzed modification of macro

1992-04-16

1994-09-27

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Enzyme treated

4351731, 422 21, C12N 1300, A61L 200

Patent

active

053506863

ABSTRACT:
A method of enhancing the enzyme-catalyzed modification of macromolecules comprising exposure of the enzyme and its macromolecule substrate to microwaves is disclosed. A frequency of from 1800 to 3000 MHz and a power of from 50 to 100 watts is preferred. The procedure is rapid, efficient, and suitable for many enzyme-catalyzed reactions. The method can be used for restriction endonuclease digestion of DNA samples and facilitates one of the time-consuming procedures of nucleic acid research. The method is also adaptable for automated procedures in biological research.

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