Microvessel cell isolation apparatus

Details Microvessel cell isolation apparatus Microvessel cell isolation apparatus

1993-07-01

1995-04-25

Beisner, William H.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435311, 422101, 422102, 422104, 494 36, C12M 142

Patent

active

054098335

ABSTRACT:
A processing vessel for isolating microvessel endothelial cells from liposuctioned fat tissues includes a fat-receiving basket defined by polyester screen material. Fat tissue removed from a patient by liposuction is received into the basket and is rinsed and digested with an enzymatic solution. The freed microvessel endothelial cells from the fat tissues are separated from the fat cells, and from blood cells and other materials which may be present in the basket by centrifuging. A bottom chamber of the processing vessel is configured to define a "pellet" of isolated endothelial cells which may be removed from the processing vessel for deposition on the inner lumenal surface of a synthetic graft which the fat-donor patient is to receive.

REFERENCES:
patent: 2910406 (1959-10-01), Novak
patent: 4423145 (1983-12-01), Stampfer et al.
patent: 4670394 (1987-06-01), Pollard et al.
patent: 4981596 (1991-01-01), Shiino et al.
patent: 5035708 (1991-07-01), Alchas et al.
patent: 5038958 (1991-08-01), Dreier
Ace Scientific Supply Co., Inc. E. Brunswich, N.J. 1983 Catalog pp. 557-559.

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