Microscope for quantitative determination of substance in specim

Details Microscope for quantitative determination of substance in specim Microscope for quantitative determination of substance in specim

1977-09-20

1981-05-26

Rosenberger, Richard A.

Optics: measuring and testing

For light transmission or absorption

356219, 356226, G01J 342

Patent

active

042695171

ABSTRACT:
A microscope comprises a full field illumination unit, a spot illumination unit using monochromatic light, means for adjusting the area of the spot region being illuminated, a light receptor for receiving light which is transmitted through a specimen, and an arithmetic unit connected with the light receptor for photometry. Full field and spot illuminations are activated simultaneously, and the position of a specimen is brought into alignment with a spot illuminated region while viewing the specimen. The area of the spot region being illuminated is adjusted so that it is substantially in conformity to the outline of the specimen. The arithmetic unit effects a quantitative determination of the total amount of light transmission through the specimen on the basis of an output from the light receptor and the area of the spot illuminated region.

REFERENCES:
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patent: 3711209 (1973-01-01), Caspersson et al.
patent: 3718400 (1973-02-01), Yonekubo
patent: 3740147 (1973-06-01), Kallet
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patent: 3843265 (1974-10-01), Egli et al.
patent: 3851949 (1974-12-01), Kraft et al.
patent: 4071299 (1978-01-01), Amano et al.
Lison, L., "Histochemistry and Cytochemistry: Theory and Methods"; Translated into Japanese by Tadashi Imaizumi, published by Hakusui Sha Book Publishing Company, pp. 60-63 (1960).

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