Microscope for performing multiple frequency fluorometric...

Radiant energy – Luminophor irradiation

Reexamination Certificate

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C250S459100, C356S317000, C356S318000

Reexamination Certificate

active

07317194

ABSTRACT:
An optical imager, such as a microscope for performing multiple frequency fluorometric measurements comprising a light source, such as a laser source is disclosed. The system is used to excite a sample into the fluorescent state. Light from the excited sample is collected by a microscope. The microscope utilizes conventional confocal optics optimized to have a very narrow depth of field, thus limiting the information collected to a thin planar region. Measurements are taken over the fluorescence lifetime of the sample simultaneously from the excitation source and from the excited sample. Information is taken in a matrix and comparison of the image matrix and the standard during simultaneous measurements yields output information.

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