Microscope for epi fluorescence and total internal...

Optical: systems and elements – Compound lens system – Microscope

Reexamination Certificate

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C359S368000, C359S629000

Reexamination Certificate

active

07405874

ABSTRACT:
The invention relates to a microscope for conventional fluorescence microscopy (Epi fluorescence) and for total internal reflection microscopy, including at least one light source (1) for the conventional fluorescence illumination and at least one light source (2) for the evanescent illumination, and including an objective (4), wherein the illuminating light coming from the light sources (1, 2) on different illumination paths (5, 6) enters the objective (4) via a beam combiner (7) and from there is passed to the sample (8), characterized in that the exit pupil of the objective (4) is imaged on the beam combiner (7) and the beam combiner is structured such that it guides the illuminating light used for the conventional fluorescence illumination and the illuminating light used for the evanescent illumination into the objective (4) along beam paths (5, 6) which are geometrically separate from one another, and preferably run parallel or coaxially to one another.

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patent: WO 2005/029149 (2005-03-01), None

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