Microscale sorting cytometer

Details Microscale sorting cytometer Microscale sorting cytometer

2005-01-31

2008-09-16

Noguerola, Alex (Department: 1753)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S643000

Reexamination Certificate

active

07425253

ABSTRACT:
The present invention provides a device and methods of use thereof in microscale cell sorting. This invention provides sorting cytometers, which trap individual cells within vessels following exposure to dielectrophoresis, allow for the assaying of trapped cells, such that a population is identified whose isolation is desired, and their isolation.

REFERENCES:
patent: 4661451 (1987-04-01), Hansen
patent: 6448794 (2002-09-01), Cheng et al.
patent: 6716642 (2004-04-01), Wu et al.
patent: 6730516 (2004-05-01), Jedrzejewski et al.
patent: 6838056 (2005-01-01), Foster
patent: 6916541 (2005-07-01), Pantano et al.
Voldman et al. (“A Microfabricated-Based Dynamic Array Cytometer,” Anal. Chem. 2002, 74, 3984-3990).
Chaudhari, A. M., Woudenberg, T. M., Albin, M. & Goodson, K. E. Transient liquid crystal thermometry of microfabricated PCR vessel arrays. Journal of Microelectromechanical Systems 7, 345-355 (1998).
Fujii, G., Tsuchiya, R., Ezoe, E. & Hirohashi, S. Analysis of nuclear localization signals using a green fluorescent protein-fusion protein library. Exp Cell Res 251, 299-306. (1999).
Guet, C. C., Elowitz, M. B., Hsing, W. & Leibler, S. Combinatorial synthesis of genetic networks. Science 296, 1466-70. (2002).
Lippincott-Schwartz, J., Snapp, E. & Kenworthy, A. Studying protein dynamics in living cells. Nat Rev Mol Cell Biol 2, 444-56. (2001).
Peelle, B. et al. Intracellular protein scaffold-mediated display of random peptide libraries for phenotypic screens in mammalian cells. Chem Biol 8, 521-34. (2001.
Rolls, M. M. et al. A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. J Cell Biol 146, 29-44. (1999).
Rutter, G. A., Kennedy, H. J., Wood, C. D., White, M. R. H. & Tavare, J. M. Real-time imaging of gene expression in single living cells. Chemistry & Biology 5, R285-R290 (1998).
Shioda, T., Andriole, S., Yahata, T. & Isselbacher, K. J. A green fluorescent protein-reporter mammalian two-hybrid system with extrachromosomal maintenance of a prey expression plasmid: application to interaction screening. Proc Natl Acad Sci U S A 97, 5220-4. (2000).
Taylor, D. L., Woo, E. S. & Giuliano, K. A. Real-time molecular and cellular analysis: the new frontier of drug discovery. Current Opinion in Biotechnology 12, 75-81 (2001).
Ting, A. Y., Kain, K. H., Klemke, R. L. & Tsien, R. Y. Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells. Proc Natl Acad Sci U S A 98, 15003-8. (2001).
Tsien, R. Y. The green fluorescent protein. Annual Review of Biochemistry 67, 509-544 (1998).
Voldman, J., Braff, R. A., Toner, M., Gray, M. L. & Schmidt, M. A. Holding Forces of Single-Particle Dielectrophoretic Traps. Biophys. J. 80, 531-541 (2001).
Voldman, J., Toner, M., Gray, M. L. & Schmidt, M. A. A Microfabrication-Based Dynamic Array Cytometer. Analytical Chemistry 74, 3984-3990 (2002).
Zhang, J., Campbell, R. E., Ting, A. Y. & Tsien, R. Y. Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol 3, 906-18 (2002).
Archer, et al (1999) “Cell Reactions to Dielectrophoretic Manipulation.” Biuochemical and Biophysical Research Communications 257, 687-698.
Chiou, et al (2005) “Massively parallel manipulation of single cells and microparticles using optical images.” Nature vol. 436 doc. 10.1038.
Fiedler, et al (1998) “Dielectrophoretic Sorting of Particles and Cells in a Microsystem.” Anal. Chem. 70, 1909-1915.
Fuhr, et al (1994) “Cell manipulation and cultivation under a.c.electric field influence in highly conductive culture media.” Biochimica et Biophysica Acta 1201 353-360.
Gray, et al (2004) “Dielectrophoretic Registration of Living Cells to a microelectrode array.” Biosensors and Bioelectronics 19, 771-780.
Ramos, et al (1998) “An electrokinetics: A review in forces in microelectrode structures.” J.Phys. D. Appl. Phys 31, 2338-2353.
Rosenthal, et al (2005) “Dielectrophoretic traps for single-particle patterning.” Biophysical Journal vol. 88 2193-2205.
Rosenthal, et al (2006) “Quantitive modeling of Dielectrophoretic traps.” Paper.
Taff, et al (2005) “A Scalable Addressable Positive-Dielectrophoretic Cell-Sorting Array.” Anal. Chem. 77 7976-7983.
Zimmerman, et al (2000) “Electromanipulation of Mammalian Cells: Fundamentals and Application.” IEEE Transactions on Plasma Science vol. 28 No. 1.
Grimm, et al (2004) The Art and Design of Genetic Screens:Mammilian Culture Cells. Nature Reviews: Genetics 5, 179-189.
Moffat, et al (2006) “Building Mammalian Signaling Pthways with RNAi Screens.” Nature Reviews: Molecular Cell Biology 7, 177-187.
Kholodenko, et al (2006) “Cell Signaling Dynamics in Time and Space.” Nature Reviews: Molecular Cell Biology 7, 165-175.
Kiger, et al (2003) “A Functional Genomic Analysis of Cell Morphology Using RNA Interference.” Journal of Biology 2:27.
Manaresi, et al (2003) “A CMOS Chip for Individual Cell Manipulation and Detection.” IEEE Journal of Solid State Circuts 38: 12 2297-2305.
Suehiro, et al (1998) “The Dielectrophoretic Movement and Positioning of a Biological Cell Using a Three Dimensional GridElectrode Syatem.” Journal Phys. D: Appl. Phys. 31 3298-3305.
Perlman, et al (2004) “Drug profiling by Automated Microscopy.” Science 306 1194-1198.
Glasser, et al (1998) “Cultivation of Cells under strong ac electric field differentiation between heating and trans-membrane potential effects.” Bioelectrochemistry and Bioenergenics 47 301-310.

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