Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-09-14
2009-06-23
Jung, Unsu (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C436S052000, C436S053000, C436S514000, C436S518000, C436S164000, C436S172000, C436S180000, C422S081000, C422S082000, C422S082080, C422S105000
Reexamination Certificate
active
07550267
ABSTRACT:
An improved microscale diffusion immunoassay utilizing multivalent reactants is disclosed. In particular, a method for detecting the presence of analyte particles in an analyte fluid is disclosed, the method comprising: (a) providing the analyte fluid comprising the analyte particles; (b) providing a diffusion fluid comprising binding particles capable of binding with the analyte particles; (c) flowing the analyte fluid and the diffusion fluid in adjacent laminar flow through a microfluidic channel; (d) allowing the analyte particles to diffuse into the diffusion fluid and bind with the binding particles to form analyte/binding particle complexes; and (e) detecting the presence of the analyte particles and the analyte/binding particle complexes, wherein each of the binding particles is capable of binding with more than one analyte particle, and wherein each of the analyte particles is capable of binding with more than one binding particle. Methods for determining the concentration of the analyte particles are also disclosed.
REFERENCES:
patent: 3449938 (1969-06-01), Giddings
patent: 3795489 (1974-03-01), Warnick et al.
patent: 4147621 (1979-04-01), Giddings
patent: 4214981 (1980-07-01), Giddings
patent: 4250026 (1981-02-01), Giddings et al.
patent: 4683212 (1987-07-01), Uffenheimer
patent: 4726929 (1988-02-01), Gropper et al.
patent: 4737268 (1988-04-01), Giddings
patent: 4756884 (1988-07-01), Hillman et al.
patent: 4830756 (1989-05-01), Giddings
patent: 4849340 (1989-07-01), Oberhardt
patent: 4894146 (1990-01-01), Giddings
patent: 4908112 (1990-03-01), Pace
patent: 5007732 (1991-04-01), Ohki et al.
patent: 5039426 (1991-08-01), Giddings
patent: 5141651 (1992-08-01), Giddings
patent: 5156039 (1992-10-01), Giddings
patent: 5193688 (1993-03-01), Giddings
patent: 5240618 (1993-08-01), Caldwell et al.
patent: 5250263 (1993-10-01), Manz
patent: 5288463 (1994-02-01), Chemelli
patent: 5304487 (1994-04-01), Wilding et al.
patent: 5389524 (1995-02-01), Larsen et al.
patent: 5439578 (1995-08-01), Dovichi et al.
patent: 5465849 (1995-11-01), Wada et al.
patent: 5480614 (1996-01-01), Kamahori
patent: 5583003 (1996-12-01), Hillyard et al.
patent: 5599432 (1997-02-01), Manz et al.
patent: 5599503 (1997-02-01), Manz et al.
patent: 5635358 (1997-06-01), Wilding et al.
patent: 5637469 (1997-06-01), Wilding et al.
patent: 5716852 (1998-02-01), Yager et al.
patent: 5726404 (1998-03-01), Brody
patent: 5726751 (1998-03-01), Altendorf et al.
patent: 5747349 (1998-05-01), van den Engh et al.
patent: 5748827 (1998-05-01), Holl et al.
patent: 5922210 (1999-07-01), Brody et al.
patent: 5932100 (1999-08-01), Yager et al.
patent: 5942443 (1999-08-01), Parce et al.
patent: 5948684 (1999-09-01), Weigl et al.
patent: 5971158 (1999-10-01), Yager et al.
patent: 5972710 (1999-10-01), Weigl et al.
patent: 5974867 (1999-11-01), Forster et al.
patent: 6067157 (2000-05-01), Altendorf
patent: 6136272 (2000-10-01), Weigl et al.
patent: 6221677 (2001-04-01), Wu et al.
patent: 6408884 (2002-06-01), Kamholz et al.
patent: 6482306 (2002-11-01), Yager et al.
patent: 6541213 (2003-04-01), Weigl et al.
patent: 2002/0090644 (2002-07-01), Weigl et al.
patent: 0 294 701 (1988-12-01), None
patent: 0 381 501 (1990-08-01), None
patent: 0 645 169 (1995-03-01), None
patent: WO 93/22053 (1993-11-01), None
patent: WO 96/04547 (1996-02-01), None
patent: WO 96/12541 (1996-05-01), None
patent: WO 96/15576 (1996-05-01), None
patent: WO 97/00125 (1997-01-01), None
patent: WO 97/02357 (1997-01-01), None
patent: WO 97/39338 (1997-10-01), None
patent: WO 98/43066 (1998-10-01), None
patent: WO 99/17100 (1999-04-01), None
patent: WO 99/17119 (1999-04-01), None
patent: WO 99/60397 (1999-11-01), None
Booker, H.E. et al, “Enzymatic Immunoassay vs. Gas/Liquid Chromatography for Determination of Phenobarbital and Diphenylhydantoin in Serum,”Clin. Chem. 21:1766-1768, 1975.
Brody et al., “Biotechnology at Low Reynolds Numbers,”Biophysical Journal71:3430-3441, Dec. 1996.
Brody, J.P. et al., “Diffusion-based extraction in a microfabricated device,”Sensors and Actuators A(Physical) A58(1):13-18, 1997.
Brody, J.P. et al., “Low Reynolds Number Micro-Fluidic Device,”Solid State Sensor&Actuator Workshop, Hilton Head, SC, pp. 105-108, Jun. 2-6, 1996.
Chiem, N. et al., “Microchip-Based Capillary Electrophoresis for Immunoassays: Analysis of Monoclonal Antibodies,”Anal. Chem. 69:373-378, Feb. 1997.
Chmelík, Josef, “Isoelectric focusing field-flow fractionation,”J. Chromatography545(2) 349-358, 1991.
Darling et al., “Integration of microelectrodes with etched microchannels for in-stream electrochemical analysis,” μTAS'98, Banff, Canada, pp. 105-108, Oct. 1998.
de Alwis et al., “Rapid Heterogeneous Competitive Electrochemical Immunoassay for IgG in the Picomole Range,”Anal. Chem. 59:2786-2789, 1987.
Ekins, R., “Immunoassay: recent developments and future directions,”Nuclear Medicine and Biology21(3):495-521, 1994.
Faucheux, L. P. et al., “Optical Thermal Ratchet,”Phys. Rev. Letters74(9):1504-1507, Feb. 1995.
Fuh et al., “Rapid Diffusion Coefficient Measurements Using Analytical SPLITT Fractionation: Application to Proteins,”Anal. Biochem. 208:80-87, 1993.
Giddings, J.C. et al. (1983), “Outlet Stream Splitting for Sample Concentration in Field-Flow Fractionation,”Separation Science&Technology18(3):293-306.
Giddings, J.C., “Field-Flow Fractionation: Analysis of Macromolecular, Colloidal and Particulate Materials,”Science260:1456-1465, Jun. 4, 1993.
Giddings, J.C., “Optimized Field-Flow Fractionation System Based on Dual Stream Splitters,”Anal. Chem. 57(4):945-947, Apr. 1985.
Griffiths et al., “Human anti-self antibodies with high specificity from phage display libraries,”EMBO J. 12(2):725-734, 1993.
Hatch, A. et al., “A rapid diffusion immunoassay in a T-sensor,”Nature Biotechnology 19(5): 461-465, May 2001.
Hawkins, K.R. et al., “Diffusion immunoassay for protein analytes,” in2ndAnnual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine&Biology, May 2-4, 2002, pp. 535-540.
Hawkins, K.R. et al., “The aggregation of multivalent immune complexes expands the useful analyte size range of the diffusion immunoassay,”Micro Total Analysis Systems 2004, vol. 1, Royal Society of Chemistry, Cambridge, U.K., 2004, pp. 129-131.
Hicks, J.M., “Fluorescence Immunoassay,”Human Pathology15(2):112-116, Feb. 1984.
Kamholz et al., “Quantitative Analysis of Molecular Interaction in a Microfluidic Channel: The T-Sensor,”Anal. Chem. 71(23):5340-5347, Dec. 1999.
Leff, H.S. et al., “Resource letter MD-1: Maxwell's demon,”Am. J. Physics58(3):201-209, 1990.
Manz, A. et al., “Planar chips technology for miniaturization of separation systems: A developing perspective in chemical monitoring,”Advances in Chromatography33:2-66, 1993.
McGregor et al., “Polarisation Fluoroimmunoassay of Phenytoin,”Clin. Chim. Acta83:161-166, 1978.
Montgomery et al., “Determination of Diphenylhydantoin in Human Serum by Spin Immunoassay,”Clin. Chem.21(2):221-226, 1975.
Paxton, J.W. et al., “Production and Characterisation of Antisera to Diphenylhydantoin Suitable for Radioimmunoassay,”J. Immunol. Methods10:317-327, 1976.
Petersen, K.E., “Silicon as a Mechanical Material,”Proc. IEEE70(5):420-457, May 1982.
Porstmann, T. et al., “Enzyme immunoassay techniques,”J. Immunol. Methods150:5-21, 1992.
Reisman, A. et al., “The Controlled Etching of Silicon in Catalyzed Ethylenediamine-Pyrocatechol-Water Solutions,”J. Electrochem. Soc. 126(8):1406-1415, Aug. 1979.
Rousselet, J. et al., “Directional motion of brownian particles induced by a periodic asymmetric potential,”Nature370:446-448, Aug. 11, 1994.
Shoji, S. et al., “Microflow devices and systems,”J. Mi
Hawkins Kenneth R.
Yager Paul
Canady Karen S.
canady + lortz LLP
Jung Unsu
University of Washington
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