Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-03-27
1999-02-23
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 231, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
058742174
ABSTRACT:
Methods of genotyping canines by analysis of polymorphisms in the number of microsatellite DNA repeats are provided. The internal repeat sequence is amplified by the use of specific primers. The number of repeats, and therefore the distance between the primers, is highly variable in a population, thereby providing an allelic marker for the locus. The combined information from multiple loci provides a means of distinguishing individuals, even among inbred dog breeds, for parentage testing, forensic testing and analysis of individuals relatedness.
REFERENCES:
Shibuya et al., "Two Polymorphic Microsatellites in a Coding Segment of the Canine Androgen Receptor Gene," Animal Genetics 24:345-348 (1993).
Fredholm et al., "Variation of Short Tandem Repeats Within and Between Species Belonging to the Canidae Family," Mammalian Genome 6:11-18 (1995).
Zajc et al., "A New Method of Paternity Testing for Dogs, Based on Microsatellite Sequences," The Veterinary Record 135: 545-547 (Dec. 3, 1994).
Francisco et al. Mammalian Genome 7(5) 359-362, 1996.
Rothuisen et al. theoretical. Appli. Gene. 89: 403-406, 1994.
Edwards et al. Amer. J. of Human Genet 49: 746-756, 1991.
Primmer te al. Anim. Genet 24:332, 1993.
Dvorak Jan
Halverson Joy
Stevenson Tamara
Bortner Scott R.
Jones W. Gary
Rees Dianne
The Perkin-Elmer Corporation
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