MicroRNA molecules

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Reexamination Certificate

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C536S023100, C536S024500

Reexamination Certificate

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10490955

ABSTRACT:
InCaenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as “small temporal RNAs” (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs, (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRNA, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.

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Pasquinelli Amy F. et al., “Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA”, NATURE, vol. 408, No. 6808, 2000, pp. 86-89.
Reinhart Brenda J. et al., “The 21-nucleotide let-7 RNA regulates development timing inCaenorhabditis elegans.”, NATURE, vol. 403, No. 6772, Feb. 24, 2000, pp. 901-906.
Moss Eric G. et al., “The cold shock domain protein LIN-28 controls developmental timing inC. elegansis regulated by the lin-4 RNA”., CELL, vol. 88, No. 5, 1997, pp. 637-646.

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