Micropropagation and production of phytopharmaceutical plants

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se

Reexamination Certificate

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C435S800000

Reexamination Certificate

active

07005298

ABSTRACT:
The development of an in vitro regeneration system that utilizes a plant growth regulator having cytokinin activity for the induction of de novo shoots or somatic embryos on explants of phytopharmaceutical plants is provided. Transfer of the regenerated shoots or somatic embryos into a solid or liquid medium with no plant growth regulators results in the rapid and prolific growth of viable plantlets. The method and its modifications are intended for application to all phytopharmaceutical plants, in particular St. John's wort (Hypericum perforatumcv. Anthos), Huang-qin (Scutellaria baicalensis),Echinaceasp., Feverfew (Tanacetum parthenium), garlic (Alliumsp.) and the like. Furthermore, a process for the uptake of nutrients, minerals or additives from the growth medium and accumulation of these in the consumable biomass of plants, hereafter referred to as phytofortification, is also described. This process provides additives within a bioavailable form within plants and renders nutrients and additives amenable for easy assimilation by the human of livestock digestive systems.

REFERENCES:
patent: 5869340 (1999-02-01), Shetty
Hypericum Perforatum In vitro culture and the production of Hypericin and other secondary Metabolites Cellarove et al.
Experiments in Plant Tissue Culture. Third Edition. John H. Dodds. Lorin W. Roberts.
Stojakowska, Anna; “Production of parthenolide in organ cultures of feverfew”; XP-000937973;Plant CH Tissue and Organ Culture,vol. 47, 1997, pp. 159-162.
Banerjee, Suchitra; “In vitro multiplication ofCentella asiatica, a medicinal herb from leaf explants”; XP-000937832; vol. 76, No. 2; Jan. 1999; pp. 147-148.
Brutovska et al.; “Cytogenetic variablility of in vitro regeneratedHypericum perforatumL. plants and their seed progenies”;Plant Science,; XP000938218; pp 221-229.
Database Biosis Online; Norton; “In-vitro propagation of Ericaceae; a comparison of the activity of cytokinins . . . ”; XP-002148178; Scientia & Horiculturae, vol. 27, pp 335-340 (abstract).
Database Biosis Online; Cocker et al.; “In vitro culture ofEchinacea purpurea”; XP-002148179; Phytopatology; vol. 87; (abstract).
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Potrykus, I. et al., “Protoplasts: Isolation, Culture, Plant Regeneration”,Methods for Plant Molecular Biology,Academic Press, Inc., Chap. 23, pp. 355-383 (1988).
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