Microporous filtration based dot immunoassay device for...

Details Microporous filtration based dot immunoassay device for... Microporous filtration based dot immunoassay device for...

2003-03-20

2008-09-02

Warden, Jill (Department: 1797)

Chemical apparatus and process disinfecting, deodorizing, preser

Control element responsive to a sensed operating condition

C422S091000, C422S105000, C435S006120, C436S180000

Reexamination Certificate

active

07419640

ABSTRACT:
The present invention is based on rapid displacement of solvents under mild vacuum in solution-solid phase reaction of immunocomplex [Ijsselmuiden et al., J. Immunol. Methods, 6 (1989): 35]. In the present invention, this is achieved by a microporous absorbing pad upon which a nitrocellulose transfer membrane is placed. The absorbing pad under mild vacuum generated/regulated by running tap effectively filters out the unbound ligand and rinsing solutions through transfer membrane, thus enhancing the reaction kinetics of immunocomplex. This mechanism, in turn reduces the incubation steps of antibody-antigen reaction from hours to few minutes, allowing total assay time in less than 20 minutes.

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Ijsselmuiden, O.E. et al. “Optimizing the solid-phasxe immunofiltration assay; A rapid alternative to immunoassays”,J. Of Immunological Methods, 119 (1989) 35-43.

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