Microporous article having a stabilized specific binding reagent

Details Microporous article having a stabilized specific binding reagent Microporous article having a stabilized specific binding reagent

1988-06-13

1992-03-10

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436524, 436525, 436535, 436808, 436824, 422 61, G01N 33543

Patent

active

050949624

ABSTRACT:
A water-insoluble microporous article comprises a microporous substrate having first and second outer surfaces. Affixed to at least one of those surfaces is a stabilized specific binding reagent admixed with certain hydrophilic, neutral or positively-charged binder materials. Particularly useful binder materials include certain quaternary polymers, vinylpyrrolidone polymers and acrylamide polymers. In this mixture, the reagent exhibits improved keeping stability compared to similar reagents used without binder materials. The reagent comprises water-insoluble particles to which are attached receptor molecules to a target ligand. Substantially none of the reagent is entrapped within the microporous substrate. This article is useful for the detection of a target ligand in an assay involving the specific binding reaction of the ligand with corresponding receptor molecules, and can be included in a diagnostic test kit. It is particularly useful for the detection of Streptococcal antigen in a biological specimen when the receptor molecules are antibodies to that antigen.

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patent: 4853335 (1989-08-01), Olsen et al.
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