Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of culturing cells in suspension
Patent
1998-03-20
2000-02-22
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of culturing cells in suspension
435 2, 4352971, 4352975, 4353051, 4353054, 4353092, 435325, 435366, 435395, 435401, 435243, C12N 500, C12N 100, C12C 100, A01N 102
Patent
active
060279389
ABSTRACT:
A patient replica is created from a layered culture medium where solid culture medium is formed so that a discontinuity exists between the layers. An infusion port is provided in registration with the discontinuity so that a fresh unadulterated sample of patient blood can be infused into the discontinuity to form a thin layer of blood between the layers of culture medium. The thin layer obviates the requirement for any anticoagulant allowing blood-borne pathogens to be readily cultured without using broth. Further antibiotics or other drug samples may be placed on the surface of the culture medium above the blood layer so that the antibiotic can diffuse through the culture medium and reveal the sensitivities of the cultured pathogens. Other samples of pathogens or tissues can be placed on the surface of the culture medium so that effects of drugs or growth factors present in the patient blood can be observed thereby allowing the entrapped blood layer to act as a biological replica of the patient.
REFERENCES:
patent: 2144255 (1939-01-01), Carpenter
patent: 3692493 (1972-09-01), Terasaki
patent: 3928142 (1975-12-01), Smith
patent: 4053362 (1977-10-01), Sforza
patent: 4187861 (1980-02-01), Heffernan
patent: 4282317 (1981-08-01), Roth
patent: 4421849 (1983-12-01), Breuker
patent: 4485171 (1984-11-01), Ikeda
patent: 4492634 (1985-01-01), Villa-Real
patent: 4678753 (1987-07-01), Hempel et al.
patent: 5482711 (1996-01-01), Medenica
patent: 5827681 (1998-10-01), Krug et al.
Millar et al. Brit. J. Haematol. vol. 69 (2), pp. 197-203, abstract enclosed, 1988.
Goldberg et al. Am. J. Clin. Path. vol. 74 (6), pp. 771-775, abstract enclosed, 1980.
Barnes Allen C.
Barnes Janice S.
Kirchanski, Esq. Stefan J.
Lankford , Jr. Leon B.
Tate Christopher R.
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