Microparticles with enhanced covalent binding capacity and...

Details Microparticles with enhanced covalent binding capacity and... Microparticles with enhanced covalent binding capacity and...

2007-04-03

2010-06-08

Desai, Anand U (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

C536S023100, C514S002600, C435S006120

Reexamination Certificate

active

07732575

ABSTRACT:
Disclosed are proteins which are covalently bound to a solid support at a first temperature where they have a first configuration, and then biomolecules are attached to the bound proteins at a higher temperature at which the proteins have more exposed functional groups, each such group being capable of covalently bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, including an amine functionalized oligonucleotide. The proteins can include, BSA (Bovine Serum Albumin) which can be bound using a reaction with the surface of a tosyl-activated microparticle.

REFERENCES:
patent: 2002/0155481 (2002-10-01), Hirota et al.
Olejnik et al, “Photocleavable biotin phosphoramidite for 5′-end-labeling, purification & phosphoryiation of oligonucleotides”,Nucleic Acids Research 1996, vol. 24, 2:361-366.
Boyd et al., “Tosyl Chloride activation of a rayon /polyester cloth for protein immobilization”, Biotechnology Techniques, Apr. 1993, vol. 7, 4:277-282.

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