Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Mutation employing a chemical mutagenic agent
Reexamination Certificate
2011-07-05
2011-07-05
Weber, Jon P (Department: 1657)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Mutation employing a chemical mutagenic agent
C435S041000, C435S134000, C435S171000, C435S254100, C435S254110, C435S441000, C435S449000, C435S454000
Reexamination Certificate
active
07972855
ABSTRACT:
There are provided microorganisms, for example,Mortierella alpina, having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.
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Akimoto Kengo
Kawashima Hiroshi
Shimizu Sakayu
Drinker Biddle & Reath LLP
Srivastava Kailash C
Suntory Holdings Limited
Weber Jon P
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