Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2007-11-06
2007-11-06
Saidha, Tekchand (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S183000, C435S189000, C435S193000, C435S195000, C435S252300, C435S320100, C530S350000, C536S023200
Reexamination Certificate
active
10614333
ABSTRACT:
The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
REFERENCES:
patent: 5518906 (1996-05-01), Hikichi et al.
patent: 5529908 (1996-06-01), Palva et al.
patent: 5932457 (1999-08-01), Moriya et al.
patent: 6171845 (2001-01-01), Elischweski et al.
patent: 6177264 (2001-01-01), Eggeling et al.
patent: 6184006 (2001-02-01), Rieping et al.
patent: 6184007 (2001-02-01), Dusch et al.
patent: 6689592 (2004-02-01), Rieping et al.
patent: 6787334 (2004-09-01), Elischweski et al.
patent: 0493060 (1992-07-01), None
patent: 0590857 (1994-04-01), None
patent: 1006189 (2000-06-01), None
patent: 1001027 (2004-05-01), None
patent: WO-97/10340 (1997-03-01), None
patent: WO-98/18954 (1998-05-01), None
patent: WO-01/83799 (2001-11-01), None
Teller, Joel H. et al., “Ketopantoate Hydroxymethyltransferase, I. Purification and Role in Pantothenate Biosynthesis,”The Journal of Biological Chemistry, vol. 251(12):3780-3785 (Jun. 1976).
Powers, Sue et al., “Ketopantoate Hydroxymethyltransferase, II. Physical, Catalytic, and Regulatory Properties,”The Journal of Biological Chemistry, vol. 251(12):3786-3793 (Jun. 1976).
Genschel, Ulrich et al., “The final step of pantothenate biosynthesis in higher plants: cloning and characterization of pantothenate synthetase fromLotus japonicusandOryza sativum(rice),”Biochem, J., vol. 341:669-678 (1999).
Sahm, Hermann et al., “D-Pantothenate Synthesis inCorynebacterium glutamicumand Use of panBC and Genes Encoding L-Valine Synthesis for D-Pantothenate Overproduction,”Applied and Environmental Microbiology, vol. 65(5):1973-1979 (1999).
Holmes, William B. et al., “Cloning and Characterization of Methenyltetrahydrofolate Synthetase fromSaccharomyces cerevisiae,” The Journal of Biological Chemistry, vol. 277(23):20205-20213 (2002).
Song, Woo-Joo et al., “Kinetics and Regulation of Pantothenate Kinase fromEscherichia coli,” The Journal of Biological Chemistry, vol. 269(43):27051-27058 (1994).
Dusch, Nicole et al., “Expression of theCorynebacterium glutamicumpanD Gene Encoding L-Aspartate-α-Decarboxylase Leads to Pantothenate Overproduction inEscherichia coli,” Applied and Environmental Micobiology, vol. 65(4):1530-1539 (1999).
Ottenhof, Harald H. et al., “Organisation of the pantothenate (vitamin B5) biosynthesis pathway in higher plants,”The Plant Journal, vol. 37:61-72 (2003).
Baigori, Mario et al., “Isolation and Characterization ofBacillus subtilisMutants Blocked in the Synthesis of Pantothenic Acid,”Journal of Bacteriology, vol. 173(13):4240-4242 (1991).
Downs, Diana M., “Evidence for a New, Oxygen-Regulated Biosynthetic Pathway for the Pyrimidine Moiety of Thiamine inSalmonella typhimurium,” Journal of Bacteriology, vol. 174(5):1515-1521 (1992).
Downs, Diana M. et al., “apbA, a New Genetic Locus Involved in Thiamine Biosyntiesis inSalmonella typhimurium,” Journal of Bacteriology, vol. 176(16):4858-4864 (1994).
Elischewski, Frank et al., “Pantothenate production inEscherichia coliK12 by enhanced expression of thepanEgene encoding ketopantoate reductase,”Journal of Biotechnology, vol. 75:135-146 (1999).
Frodyma, Michael E. et al., “ApbA, the Ketopantoate Reductase Enzyme ofSalmonella typhimuriumIs Required for the Synthesis of Thiamine via the Alternative Pyrimidine Biosynthetic Pathway,”The Journal of Biological Chemistry, vol. 273(10):5572-5576 (1998).
Frodyma, Michael E. et al., “The panE Gene, Encoding Ketopantoate Reductase, Maps at 10 Minutes and Is Allelic toapbAinSalmonella typhimurium,” Journal of Bacteriology, vol. 180(17):4757-4759 (1998).
Merkel, William K. et al., “Characterization and sequence of theEscherichia coli panBCDgene cluster,”FEMS Microbiology Letters, vol. 143(2)-3):247-252 (1996).
Primerano, Donald A. et al., “Role of Acetohydroxy Acid Isomeroreductase in Biosynthesis of Pantothenic Acid inSalmonella typhimurium,” Journal of Bacteriology, vol. 153(1):259-269 (1983).
Hermann Theron
Patterson Thomas A.
Pero Janice G.
Yocum R. Rogers
BASF - Aktiengesellschaft
Fronda Christian L.
Lahive & Cockfield LLP
Saidha Tekchand
LandOfFree
Microorganisms and processes for enhanced production of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Microorganisms and processes for enhanced production of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microorganisms and processes for enhanced production of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3854272