Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-03-21
1992-01-07
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 30, 435 34, 435296, 435805, 435810, C12Q 118, C12Q 124, C12Q 104, C12M 124
Patent
active
050791443
ABSTRACT:
A test for microbial growth is carried out by culturing a microorgnism to be tested in a lliquid culture medium containing an enzymatically hydrolyzable fluorogenic substrate for a period of less than about 7 hours, measuring fluorescence of the culture medium resulting from hydrolysis of the fluorogenic substrate by growth of the microorganism, continuing culturing of the microorganism in the culture medium for an additional period, and measuring microorganism growth by a non-fluorescent method as a confirmatory test of growth determined from fluorescence. Preferably, the additional culture period is overnight and the non-fluorescent method is by visual inspection. The culture medium may be in the wall of a microtitre plate, and the culture medium can contain an antibiotic to test for sensitivity of a microorganism to the antibiotic or to test for minimum inhibitory concentration of the antibiotic.
REFERENCES:
patent: 3415361 (1968-12-01), Adams et al.
patent: 3509026 (1967-01-01), Sanders
patent: 4066511 (1978-12-01), Montagnon
patent: 4094647 (1978-06-01), Deutsch et al.
patent: 4125376 (1978-11-01), Razulis
patent: 4234316 (1980-11-01), Hevey
patent: 4242447 (1980-12-01), Findl et al.
patent: 4378344 (1983-03-01), Zahradnik et al.
patent: 4387164 (1983-06-01), Hevey et al.
Bailey et al., 1978, Diagnostic Microbiology, the C.V. Mosby Co., Saint Louis, Mo., pp. 396-399.
Reeves et al., Antimicrobial Agents and Chemotherapy, vol. 18, Dec. 1980, pp. 844-852.
Littel et al., Applied & Environment Microbiology (1983) 45, 622-627.
Smith et al., Thrombosis Research (1980) 17, 393-402.
Grange et al., Journal of Clinical Pathology (1977) 13, 151-153.
Kilian et al., Acta Path. Microbiol. Section B (1976) 84, 245-251.
Slifkin et al., Journal of Clinical Microbiology (1983) 18, 29-32.
Feng et al., Applied & Environment Microbiology (Jun. 1982) 43, 1320-1329.
Watson, Methods in Microbiology (1976) 9, 1.
Peterson et al., Journal of Food Science (1978) 43, 1853-1856.
Bradbury, Journal of Clinical Microbiology (1977) 5, 531-534.
Godsey et al., Journal of Clinical Microbiology (1981) 13, 483-490.
Badley Robert A.
Carr Anthony H.
Jobling Ian
Sands Thomas J.
Naff David M.
Radiometer Corporate Development Ltd.
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