Microorganism producing 5′-xanthylic acid

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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Reexamination Certificate

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07456010

ABSTRACT:
The invention relates toCorynebacterium ammoniagenesCJXSP 0201 KCCM 10448 producing 5′-xanthylic acid. More specifically, the invention relates toCorynebacterium ammoniagenesCJXSP 0201 KCCM 10448 which is a mutant strain ofCorynebacterium ammoniagenesKCCM 10340 having a resistance to osmotic pressure. In order to obtain mutant strain having enhanced respiratory activity, the present invention adoptedCorynebacterium ammoniagenesKCCM 10340 as parent strain and treated it with UV radiation and mutation derivatives such as N-methyl-N′-nitro-n-nitrosoguanidine (NTG) according to ordinary procedure. Therefore,Corynebacterium ammoniagenesCJXSP 0201 KCCM 10448 of the present invention makes it possible to overcome growth-standing phase rapidly on early culture and for same period of fermentation, can accumulate 5′-xanthylic acid in culture medium at a high yield and concentration rate.

REFERENCES:
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Fujio, T., et al; “High level expression of XMP aminase inE.coliits application for the industrial production of 5′-guanylic acid”; Biosci Biotechnol Biochem Article; May 1997; 61(5):840-5;′ PubMed; National Library of Medicine; Internet—NCBI.

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