Microorganism, lipid-modifying agent, and the method of...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S253300, C435S198000

Reexamination Certificate

active

07993887

ABSTRACT:
The present invention provides a new supply source of phospholipase A1, which is useful for phospholipid modification and lipid biochemical research, and offers a method capable of efficiently manufacturing a large amount of high DHA content phospholipids and lysophospholipids. A novel microorganism HFKI-0020 (FERM AP-20545) of the genusPseudomonaswhich produces enzymes with phospholipase A1activity can be used as a new supply source of enzymes with the phospholipase A1activity. A lipid-modifying agent containing an effective dose of enzymes with the phospholipase A1activity produced by those novel microorganisms allows the efficient mass-production of lysophospholipids.

REFERENCES:
N. Tsukagohi et al. “structure ans Synthesis of a Lipid-containing Bacteriophage”, Eur. J. Biochem. 60: 603-613 (1975).
R.T. Espejo et al. “properties and Characterization of the Host Bacterium of Bacteriophage PM2”, J. Bacteriol. 95(5):1887-1891. (May 1968).
Nishihara, Masaaki, et al., “Isolation of phospholipase A1producing.strain originating from marine organisms,” Japan Society for Bioscience, Biotechnology, and Agrochemistry, Annual conference presentations database (2005), Mar. 5, 2005, p. 205, (Abstract 30D087a).
Kamata, Masazumi, et al., “Phospholipase A1produced by marine bacteria,” The 37th Oil Chemistry Symposium, Presentation overview, Sep. 16, 1998, p. 94 (Abstract 1E17).
Yazawa, Kazunaga, et al., “Phospholipase A1produced by DHA producing isolate and its use,”Journal of Lipid Nutrition, Japan Society for Lipid Nutrition, Presentation overview, Aug. 24, 1998, p. 103, vol. 7, No. 2, (Abstract 001).
Nishihara, Masaaki, et al., “Characteristics of PLA1producingPseudomonassp. HFKI0020 derived from marine organisms,” 8th Conference of Japan Society for Marine Biotechnology, May 28, 2005, p. 86, (Abstract 1-13).
ishihara, Masaaki, et al., “The hydrolytic property of phospholipase A1in a culture supernatant ofPseudomonassp. HFKI0020 strain,” Japanese Society of Enzyme Engineering, Presentation overview, Oct. 25, 2005, p. 59, (Abstract B-5).

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