Microorganism immobilized in a gel prepared from polyvinyl alcoh

Details Microorganism immobilized in a gel prepared from polyvinyl alcoh Microorganism immobilized in a gel prepared from polyvinyl alcoh

1988-07-26

1991-07-23

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435182, C12N 1110, C12N 1104

Patent

active

050343246

ABSTRACT:
A hydrated gel of high water content and high porosity containing an immobilized microorganism is prepared by dropwise addition of an aqueous solution containing a microorganism, polyvinyl alcohol and a polysaccharide to an aqueous solution containing polyvalent metal ions to gel the polysaccharide and form spherical gel beads, and subjecting the beads to at least one cycle of freezing and thawing to gel the polyvinyl alcohol. The polysaccharide can be sodium alginate and the polyvalent metal ions can be provided by calcium chloride. Freezing is at a temperature not higher than -5.degree. C., and the cycle of freezing and thawing is preferably repeated at least two times.

REFERENCES:
patent: 4138292 (1979-02-01), Chibata et al.
patent: 4617271 (1986-10-01), Nambu

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