Microorganism immobilization in a .beta.-chitin carrier

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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204403, 435182, 435288, 435817, C12N 1110, C12N 1104, C12M 140, G01N 2726

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active

053546792

ABSTRACT:
A suspension of a microorganism or a plurality of different microorganisms is added to a dispersion of .beta.-chitin gel, and the resultant dispersion is molded and dried to produce an immobilized microorganism such as a film or membrane containing the microorganism. The .beta.-chitin gel dispersion is prepared by isolating and grinding .beta.-chitin from a source such as cuttlefish pen or squid pen, dispersing the ground .beta.-chitin in water and forming a gel dispersion. In forming the gel dispersion, the dispersion of ground .beta.-chitin is gelled and the gel is dispersed in water. Freeze-thawing may be used in gelling the dispersion of ground .beta.-chitin. A film of the immobilized microorganism can be formed on an electrode to prepare a microorganism electrode.

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Ohashi et al.; "Simple and mild preparation of an enzyme-immobilized membrane for a biosensor using .beta.-type crystalline chitin"; Analytica Chimica Acta; vol. 262, No. 1; 1992; pp. 19-25.
Kovalenko et al.; "Enzyme-based glucose analyzers"; 2357 Journal of Analytical Chemistry USSR; 44(1989) Mar., No. 3, Part 1; pp. 307-316.
Portier; "Chitin immobilization systems for hazardous waste detoxification and biodegradation"; Chemical Abstracts; vol. 108; 1988; p. 293, 108:10677p.
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Okafor; "Isolation of chitin from the shell of the cuttlefish, sepia officinalis"; Chemical Abstracts; vol. 63, 1965; col. 12035.

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