Microorganism immobilization

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-5-aza-bicyclo

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435119, 435161, 435162, 435177, 435179, 435180, C12P 3506, C12P 1718, C12P 714, C12N 1102

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active

048988170

ABSTRACT:
An improved repetitive, batch-type fermentation process is provided. It includes a first, non-repetitive step of forming a support bearing a film of live and reproductive microorganisms immobilized thereon, e.g. using a cloth support. Then, the process involves the steps of freely suspending and stirring small segments of support cloth bearing a fixed film of live and reproductive microorganisms immobilized thereon within a fermenter containing suitable nutrient liquor to carry out a fermentation process, whereby the microorganisms produce a fermented liquor; withdrawing fermented liquor from the fermenter while retaining the support cloth within the fermenter; adding fresh nutrient to the fermenter containing the support cloth bearing the fixed film of live and reproductive microorganisms immobilized thereon; and repeating the above steps a plurality of times. This provides an improved batch-type fermentation process which minimizes the problems of start-up and which can be readily scaled-up and automated.

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Atkinson, et al., Trans. Instn. Chem. Engrs., vol. 50, 1972, pp. 208-216.
Atkinson, et al., Biotechnology & Bioengineering, vol. XVII, 1975, pp. 1245-1267.
Abbott, B. J., Immobilized Cells, Annual Reports on Fermentation Processes, vol. 2, Academic Press, 1928, pp. 205-208.
Olson et al., Immobilized Enzymes in Food and Microbial Processes, Plenum Press, N.Y., 1974, pp. 176-177.

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