Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1982-01-22
1985-07-02
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 38, 435 34, 435808, 435803, 436 63, 436 56, C12Q 116, C12Q 104, C12Q 110, G01N 3358
Patent
active
045268657
ABSTRACT:
An identification technique for micro-organisms in which a dilute solution of a culture medium containing an unknown micro-organism has added thereto an emissive agent such as a radioactive amino acid to produce a mix of emissive products that depends on the metabolic mechanism of the micro-organism. After a predetermined incubation period, the reaction is arrested and the solution layered onto a gel plate where it is subjected to electrophoresis. The plate is then autoradiographed by exposing the gel to a sensitive photographic film for a period sufficient to produce thereon a characteristic band pattern functioning as an identifier for the micro-organism. Identification may be effected by comparing the identifier for the unknown with a collection of identifiers for known micro-organisms to find a match with one of these known identifiers. The comparison is preferably carried out by scanning the unknown identifier to produce a signal which is compared with signals representing known identifiers stored in a computer which, when a match is found, yields identification data. Alternatively, the emissive products, after separation, may be detected by direct scanning to provide an identifier signal for computer processing.
REFERENCES:
patent: 2914447 (1959-11-01), Levin
patent: 3470373 (1969-09-01), Brewer et al.
patent: 3673410 (1972-06-01), Waite et al.
patent: 3858045 (1974-12-01), Waters
patent: 3935073 (1976-01-01), Waters
patent: 3944471 (1976-03-01), Waters
patent: 3969496 (1976-07-01), Schrot
patent: 3997404 (1976-12-01), Waters
patent: 4057470 (1977-11-01), Schrot
patent: 4142939 (1979-03-01), Morse et al.
patent: 4288543 (1981-09-01), Sielaff et al.
Tsukamura et al., "Thin Layer Chromatography after Incubation with 35 S-Methionine, Meaning of Petroleum Ether Soluble Frac." Kekkaku 55(12) (1980), pp. 525-530, Chemical Abstracts 94: 135993n.
El-Sharkawy et al., "Differentiation Among Xanthamonas Species by Polyacrylamide Gel Electrophoresis of Soluble Protein" Journal of General Microbiology 68 (1971), pp. 155-165.
Theodore et al., "Polyacrylamide Gell Electrophoresis of Bacterial L-Forms and Mycoplasma Species of Human Origin" Applied Microbiology 21(2) (2-1971), pp. 272-277.
Lotan et al., "Plasma Membranes of Eukaryotes" in Advanced Cell Biology by Schwartz et al., Van Nostrand Reinhold Co., NY (1981), pp. 129-133.
Larsen et al., "Polyacrylamide Gel Electrophoresis of Corynebacterium Diptheriae: A Possible Epidemiological Aid" Applied Microbiology 22(5) (1971), pp. 885-890.
Razin et al., "Identification of Mycoplasma and Other Microorganisms by Polyacrylamide-Gel Electrophoresis of Cell Proteins" Journal of Bacteriology 94(6) (1967), pp. 1807-1810.
Winkelman et al., "Automatic Calculation of Desitometer Scans of Electrophoretic Strips" Clinical Chemistry 15(8) 1969, pp. 708-711.
Robinson, "Starch Gel Electrophoresis of Bacterial Cell Free Extracts" Lab. Pract., 17(2), pp. 196-198, Chemical Abstracts 68: 66260v.
Flygare et al., "Electrophoretic Light Scattering" Electro-Opt., Ser. 1 (part 1) (1976), pp. 321-366.
Lawson, "Medical and Environmental Applications of Quadrupole Mass Spectrometry" Quadrupole Mass Spec., (1976), pp. 307-333, C.A. 86: 52267n.
AMB Systems Corp.
Ebert Michael
Tarcza J. E.
Warden Robert J.
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