Microorganism having the improved gene for...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing element or inorganic compound except carbon dioxide

Reexamination Certificate

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C435S170000, C435S189000, C435S252330, C530S350000, C536S023200

Reexamination Certificate

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07816109

ABSTRACT:
A hydrogen producing method by culturing a microorganism having a hydrogenase gene, in which a lactic acid biogenetic path and a succinic acid biogenetic path in anaerobic metabolic paths are inactivated, under an anaerobic condition in the presence of organic substrate. The microorganism may further have a formate dehydrogenase gene. The microorganism may beEscherichia coliwhich is a facultative anaerobic bacterium.

REFERENCES:
patent: 7432091 (2008-10-01), Yukawa et al.
patent: WO 2004/074495 (2004-09-01), None
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Kumar et al.,Redirection of biochemical pathways for the enhancement of H2production by Enterobacter cloacae, Biotechnology Letters, 23: 537-541, 2001.
Sode et al, “Metabolic Engineering Approaches for the Improvement of Bacterial Hydrogen production Based onEscherichia coliMixed Acid Fermentation”, Biohydrogen II, May 1, 2001, pp. 195-204.

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