Microorganism and process for preparing D-biotin using the same

Details Microorganism and process for preparing D-biotin using the same Microorganism and process for preparing D-biotin using the same

1993-01-13

1994-12-20

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 435119, C12N 1500, C12N 121

Patent

active

053745540

ABSTRACT:
A microorganism derived from a host microorganism capable of producing d-biotin by introducing a recombinant plasmid being incorporated with a biotin gene cloned from a microorganism of the genus Serratia capable of producing d-biotin and further integrating an exogenous biotin gene into the chromosome, and a process for preparing d-biotin which comprises cultivating the microorganism in a culture medium so that d-biotin is formed and accumulated in the culture medium and collecting the d-biotin. The microorganism of the invention has an extremely high productivity of d-biotin, and hence, d-biotin can be produced in a large amount by cultivating the microorganism of the invention.

REFERENCES:
Eisenberg et al. 1982, Antimicrobial Agents and Chemotherapy vol. 21(1):5-10.
Eisenberg, Max, 1987 "Biosynthesis of Biotin and Lipoic Acid" pp. 544-550, in F. C. Neidhart ed. Escherichia coli and Salmonella typhimurium; Cellular and Molecular Biology American Society for Microbiology, Washington, D.C.

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