Micromachined lysing device and method for performing cell...

Solid material comminution or disintegration – Processes – By operations other than force of contact with solid surface

Reexamination Certificate

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C241S002000, C241S301000, C435S259000, C435S820000

Reexamination Certificate

active

06942169

ABSTRACT:
A method and device for performing lysing on a cell-containing fluid, in which the fluid flows through a vibrating micromachined tube to physically rupture the cell walls (mechanical lysis), and/or to mix, agitate or homogenize the fluid during chemical lysis, and/or to mix, agitate or homogenize the lysate for analysis or other processing after lysing. The tube includes a freestanding portion spaced apart from a surface of a substrate on which the tube is formed. The device further includes means for vibrating the freestanding portion of the tube at a level sufficient to rupture the walls of cells in a fluid flowing through the freestanding portion (for mechanical lysing) or to mix the fluid and a chemical lysing additive within the freestanding portion (for chemical lysing).

REFERENCES:
patent: 5639423 (1997-06-01), Northrup et al.
patent: 6100084 (2000-08-01), Miles et al.
patent: 6431476 (2002-08-01), Taylor et al.
patent: 6477901 (2002-11-01), Tadigadapa et al.
Taylor, Michael, et al;Lysing Bacterial Spores by Sonication Through a Flexible Interface in a Microfluidic System, Analytical Chemistry, vol. 73, No. 3, Feb. 1, 2001, pp. 492-496.
Tzannis, Stelios T.,Tutorial on Cell Disruption.
Smith, Tiffany J.,TechNotes 5(4),Cell Disruption: Getting the RNA Out.

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