Microinjection of cryoprotectants for preservation of cells

Details Microinjection of cryoprotectants for preservation of cells Microinjection of cryoprotectants for preservation of cells

2006-08-22

2006-08-22

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of storing cells in a viable state

C435S001300, C435S002000, C435S325000, C435S366000, C436S018000

Reexamination Certificate

active

07094601

ABSTRACT:
A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering the stored biological material from storage. The invention also features a method of culturing a cell in vitro using a hypertonic medium. Carbohydrate sugars such as trehalose, sucrose, fructose, dextran, and raffinose, may be used as bio-protective agents or in the culture medium.

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