Microinjection methods to transform arthropods with exogenous DN

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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800 2, 800DIG1, 935 53, 935 63, C12N 1500, C12N 500

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057029326

ABSTRACT:
This invention relates to methods for transforming arthropods with exogenous DNA. The methods are useful for arthropods that have not previously been amenable to DNA-mediated transformation, and also provide a simpler and more efficient means of transforming arthropods than have previously been described. The method involves microinjecting a nucleic acid sequence encoding a desired trait into the reproductive tract of a female arthropod before oviposition.

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