Microfluidic protein crystallography

Details Microfluidic protein crystallography Microfluidic protein crystallography

2011-04-19

2011-04-19

Kunemund, Robert M (Department: 1714)

Single-crystal, oriented-crystal, and epitaxy growth processes;

Apparatus

C117S201000, C117S202000, C117S068000, C117S070000

Reexamination Certificate

active

07927422

ABSTRACT:
The use of microfluidic structures enables high throughput screening of protein crystallization. In one embodiment, an integrated combinatoric mixing chip allows for precise metering of reagents to rapidly create a large number of potential crystallization conditions, with possible crystal formations observed on chip. In an alternative embodiment, the microfluidic structures may be utilized to explore phase space conditions of a particular protein crystallizing agent combination, thereby identifying promising conditions and allowing for subsequent focused attempts to obtain crystal growth.

REFERENCES:
patent: 3570515 (1971-03-01), Kinner
patent: 3747628 (1973-07-01), Holster et al.
patent: 4046159 (1977-09-01), Pegourie
patent: 4119368 (1978-10-01), Yamazaki
patent: 4153855 (1979-05-01), Feingold
patent: 4245673 (1981-01-01), Bouteille et al.
patent: 4434704 (1984-03-01), Surjaatmadja
patent: 4898582 (1990-02-01), Faste
patent: 4948564 (1990-08-01), Root et al.
patent: 4992312 (1991-02-01), Frisch
patent: 5085562 (1992-02-01), Van Lintel
patent: 5088515 (1992-02-01), Kamen
patent: 5096388 (1992-03-01), Weinberg
patent: 5126115 (1992-06-01), Fujita et al.
patent: 5164558 (1992-11-01), Huff et al.
patent: 5171132 (1992-12-01), Miyazaki et al.
patent: 5224843 (1993-07-01), Van Lintel
patent: 5259737 (1993-11-01), Kamisuki et al.
patent: 5265327 (1993-11-01), Faris et al.
patent: 5290240 (1994-03-01), Horres, Jr.
patent: 5336062 (1994-08-01), Richter
patent: 5346372 (1994-09-01), Naruse et al.
patent: 5375979 (1994-12-01), Trah
patent: 5376252 (1994-12-01), Ekstrom et al.
patent: 5400741 (1995-03-01), DeTitta et al.
patent: 5423287 (1995-06-01), Usami et al.
patent: 5529465 (1996-06-01), Zengerle et al.
patent: 5593130 (1997-01-01), Hansson et al.
patent: 5637469 (1997-06-01), Wilding et al.
patent: 5642015 (1997-06-01), Whitehead et al.
patent: 5659171 (1997-08-01), Young et al.
patent: 5660370 (1997-08-01), Webster
patent: 5681024 (1997-10-01), Lisec et al.
patent: 5705018 (1998-01-01), Hartley
patent: 5759014 (1998-06-01), Van Lintel
patent: 5775371 (1998-07-01), Pan et al.
patent: 5836750 (1998-11-01), Cabuz
patent: 5842787 (1998-12-01), Kopf-Sill et al.
patent: 5875817 (1999-03-01), Carter
patent: 5876187 (1999-03-01), Afromowitz et al.
patent: 5876675 (1999-03-01), Kennedy
patent: 5885470 (1999-03-01), Parce et al.
patent: 5932100 (1999-08-01), Yager et al.
patent: 5932799 (1999-08-01), Moles
patent: 5942443 (1999-08-01), Parce et al.
patent: 6007309 (1999-12-01), Hartley
patent: 6043080 (2000-03-01), Lipshutz et al.
patent: 6123769 (2000-09-01), Sanjoh
patent: 6155282 (2000-12-01), Zachary et al.
patent: 6165694 (2000-12-01), Liu
patent: 6174365 (2001-01-01), Sanjoh
patent: 6174675 (2001-01-01), Chow et al.
patent: 6296673 (2001-10-01), Santarsiero et al.
patent: 6345502 (2002-02-01), Tai et al.
patent: 6361958 (2002-03-01), Shieh et al.
patent: 6409832 (2002-06-01), Weigl et al.
patent: 6767706 (2004-07-01), Quake et al.
patent: 7517656 (2009-04-01), Martin et al.
patent: 2001/0027745 (2001-10-01), Weigl et al.
patent: 2001/0054778 (2001-12-01), Unger et al.
patent: 2002/0037499 (2002-03-01), Quake et al.
patent: 2002/0191048 (2002-12-01), Mutz et al.
patent: 2003/0061687 (2003-04-01), Hansen et al.
patent: 2003/0096310 (2003-05-01), Hansen et al.
patent: 2004/0115731 (2004-06-01), Hansen et al.
patent: 2005/0019794 (2005-01-01), Nassef et al.
patent: 2005/0062196 (2005-03-01), Hansen et al.
patent: 2005/0229839 (2005-10-01), Quake et al.
patent: 2005/0282175 (2005-12-01), Taylor et al.
patent: 0 592 094 (1994-04-01), None
patent: 0 703 364 (1996-03-01), None
patent: 0 706 004 (1996-04-01), None
patent: 0 779 436 (1997-06-01), None
patent: 0 829 360 (1998-03-01), None
patent: 0 845 603 (1998-06-01), None
patent: 0 999 055 (2000-05-01), None
patent: 2 155 152 (1985-09-01), None
patent: 2 308 460 (1997-06-01), None
patent: WO 98/07069 (1998-02-01), None
patent: WO 99/00655 (1999-01-01), None
patent: WO 99/04361 (1999-01-01), None
patent: WO 99/17093 (1999-04-01), None
patent: WO 99/52633 (1999-10-01), None
patent: WO 00/00678 (2000-01-01), None
patent: WO 00/43748 (2000-07-01), None
patent: WO 00/60345 (2000-10-01), None
patent: WO 01/09595 (2001-02-01), None
patent: WO 01/09595 (2001-02-01), None
“Biochips,” Nature Biotechnology, vol. 18, Supplement 2000, pp. IT43-IT44, 2000.
“Chapter 9: Microfluidic Devices,” Micromachined Transducers Sourcebook, pp. 779-882, 1998.
“The Liver Chip,” Technology Review, pp. 64-67, Mar. 2003.
Abola, Enrique et al., “Automation Of X-Ray Crystallography,” Nature Structural Biology, Structural Genomics Supplement, pp. 973-977, Nov. 2000.
Andersen, Gregers Rom et al., “A Spreadsheet Approach To Automated Protein Crystallization,” Journal of Applied Crystallography, vol. 29, pp. 236-240, 1996.
Andersson, Helene et al., “Consecutive Microcontact Printing—Ligands For Asymmetric Catalysis In Silicon Channel,” Sensors and Actuators B, vol. 79, pp. 78-84, 2001.
Angell, James B. et al., “Silicon Micromechanical Devices,” Scientific American, pp. cover, 44-55, Apr. 1983.
Armani, Deniz et al., “Re-Configurable Fluid Circuits By PDMS Elastomer Micromachining,” IEEE Int. Conf. Micro Electro Mech. Syst. Tech. Digest, vol. 12, pp. 222-227, 1999.
Ballantyne, J. P. et al., “Selective Area Metallization By Electron-Beam Controlled Direct Metallic Deposition,” J. Vac. Sci. Technol., vol. 10, No. 6, pp. 1094-1097, Nov. 1973.
Belgrader, Phillip et al., “Rapid Pathogen Detection Using A Microchip PCR Array Instrument,” Clinical Chemistry, vol. 44, No. 10, pp. 2191-2194, 1998.
Benard, W. L. et al., “A Titanium-Nickel Shape-Memory Alloy Actuated Micropump,” Transducers '97, 1997 International Conference on Solid-State Sensors and Actuators, Chicago, Illinois, pp. 361-364, Jun. 16-19, 1997.
Berry, Michael B., “Protein Crystallization: Theory And Practice,” Excerpts from Doctoral Thesis, 36 pages, Sep. 17, 1995.
Black, Harvey, “Tiny Technology Promises Tremendous Profits,” The Scientist, vol. 15, No. 21, 4 pages, Oct. 29, 2001.
Bloomstein, T. M. et al., “Laser-Chemical Three-Dimensional Writing For Microelectromechanics And Application To Standard-Cell Microfluidics,” J. Vac. Sci. Technol. B, vol. 10, No. 6, pp. 2671-2674, Nov. 1992.
Bousse, Luc et al., “Electrokinetically Controlled Microfluidic Analysis Systems,” Annu. Rev. Biophys. Biomol. Struct., vol. 29, pp. 155-181, 2000.
Brechtel, R. et al., “Control Of The Electroosmotic Flow By Metal-Salt-Containing Buffers,” Journal of Chromatography A, vol. 716, pp. 97-105, 1995.
Brush, Michael, “Automated Laboratories,” The Scientist, vol. 13, No. 4, 10 pages, Feb. 15, 1999.
Bryzek, Janusz et al., “Micromachines On The March”, IEEE Spectrum, vol. 31, No. 5, pp. 20-31, May 1994.
Buchaillot, Lionel et al., “Silicon Nitride Thin Films Young's Modulus Determination By An Optical Non Destructive Method,” Jpn. J. Appl. Phys., vol. 36, Part 2, No. 6B, pp. L794-L797, Jun. 15, 1997.
Burbaum, Jonathan J. et al., “New Technologies For High-Throughput Screening,” Current Opinion in Chemical Biology, vol. 1, pp. 72-78, 1997.
Calkins, Kathryn, “Mycometrix: Rubber Chips,” BioCentury, 2 pages, Oct. 16, 2000.
Carter, Charles W. Jr. et al., “Protein Crystallization Using Incomplete Factorial Experiments,” Journal of Biological Chemistry, vol. 254, No. 23, pp. 12219-12223, Dec. 10, 1979.
Carter, Charles W. Jr. et al., “Statistical Design Of Experiments For Protein Crystal Growth And The Use Of A Precrystallization Assay,” Journal of Crystal Growth, vol. 90, pp. 60-73, 1988.
Chang, Jun Keun et al., “Functional Integration Of Serial Dilution And Capillary Electrophoresis On A PDMS Microchip,” Biotechnology and Bioprocess Engineering, vol. 8, No. 4, pp. 233-239, 2003.
Chayen, Naomi E. et al., “An Auto

Affiliated with

Also associated with

Rating

Microfluidic protein crystallography does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Microfluidic protein crystallography, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microfluidic protein crystallography will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2635611