Microfluidic platform and method of generating a gradient...

Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition

Reexamination Certificate

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Reexamination Certificate

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07470403

ABSTRACT:
An apparatus and method of using the same are provided for generating a gradient of particles within a microfluidic device. The microfluidic device includes a channel having an input and an output. The channel is filled with a predetermined fluid. Thereafter, particles from a source pass through a porous membrane into the input of the channel. A second membrane is provided adjacent the output of the channel to minimize convection therein. A sink communicates with the output of the channel. The source/sink combination creates a pseudo-steady state in the channel wherein the concentration of particles at a point does not vary dramatically with time.

REFERENCES:
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Presentation, University of Wisconsin, Madison, WI, Apr. 6, 2005, entitled “Using Surface Patterning And Microfluidics to Understand Human Embryonic Stem Cell Differentiation” by Abhyankar et al.
Paper from the Journal ofThe Royal Society Of Chemistry, dated Apr. 22, 2005, entitled “Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generator” by Walker et al.
Article fromLangmuir2000, pp. 8311-8316, entitled: “Generation of Solution and Surface Gradients Using Microfluidic Systems” by Jeon et al.
Report fromSciencevol. 287, dated Feb. 11, 2000, entitled “Polarization Of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis” by Servant et al.

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