Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-12-26
2006-12-26
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120
Reexamination Certificate
active
07153673
ABSTRACT:
In a method for performing a hot start polymerase chain reaction, a reaction mixture including a primer, a template molecule, and a buffer—but no polymerase enzyme—is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A polymerase enzyme is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the hot start polymerase chain reaction is performed. In a method for performing a temperature mediated reaction, a first component of the temperature mediated reaction is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A second component of the temperature mediated reaction is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the temperature mediated reaction is performed.
REFERENCES:
patent: 5176203 (1993-01-01), Larzul
patent: 5779868 (1998-07-01), Parce et al.
patent: 5858195 (1999-01-01), Ramsey
patent: 5863502 (1999-01-01), Southgate et al.
patent: 5942443 (1999-08-01), Parce et al.
patent: 5965001 (1999-10-01), Chow et al.
patent: 5965410 (1999-10-01), Chow et al.
patent: 6020141 (2000-02-01), Pantoliano et al.
patent: 6033546 (2000-03-01), Ramsey
patent: 6062261 (2000-05-01), Jacobson et al.
patent: 6103537 (2000-08-01), Ullman et al.
patent: 6221600 (2001-04-01), MacLeod
patent: 6300075 (2001-10-01), Preston et al.
patent: 6303343 (2001-10-01), Kopf-Sill
patent: 6537799 (2003-03-01), Chow et al.
patent: WO-94/05414 (1994-03-01), None
patent: WO-96/04547 (1996-02-01), None
patent: WO-97/02357 (1997-01-01), None
patent: WO-98/00707 (1998-01-01), None
patent: WO-98/45481 (1998-10-01), None
Chou, Q. et al., “Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications,” Nucl. Acids Res. (1992) 20(7):1717-1723.
D'Aquila, R.T. et al., “Maximizing sensitivity and specificity of PCR by pre-amplification heating,” Nucl. Acids Res. (1991) 19:3749.
Kainz, P. et al., “Specificity-Enhanced Hot-Start PCR: Addition of Double-Stranded DNA Fragments Adapted to the Annealing Temperature,” BioTechniques (2000) 28(2):278-282.
Kaijalainen, S. et al., “An alternative hot start technique for PCR in small volumes using beads of wax-embedded reaction components dried in trehalose,” Nucl. Acids Res. (1993) 21(12):2959-2960.
Kellogg, D.E. et al., “TaqStart Antibody: ‘Hot Start’ PCR Facilitated by a Neutralizing Monoclonal Antibody Directed Against Taq DNA Polymerase,” BioTechniques (1994) 16(6):1134-1137.
Manz, A. et al., “Micromachining of monocrystalline silicon and glass for chemical analysis systems,” Trends in Anal. Chem. (1991) 10(5):144-149.
Manz, A. et al., “Planar Chips Technology for Miniaturization of Separation Systems: A Developing Perspective in Chemical Monitoring,” Adv. in Chromatog. (1993) 33:1-66.
Moretti, T. et al., “Enhancement of PCR Amplification Yield and Specificity Using AmpliTaq Gold DNA Polymerase,” BioTechniques (1998) 25(4):716-722.
Caliper Life Sciences, Inc.
Horlick Kenneth R.
McKenna Donald R.
Petersen Ann C.
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