Microfluidic device for forming monodisperse lipoplexes

Details Microfluidic device for forming monodisperse lipoplexes Microfluidic device for forming monodisperse lipoplexes

2007-05-08

2010-10-12

Crouch, Deborah (Department: 1632)

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

C264S004100

Reexamination Certificate

active

07811603

ABSTRACT:
A method for forming monodisperse lipoplex assemblies includes providing a microfluidic device having a main microfluidic channel coupled to first and second reactant inlet channels. The first inlet channel is used to deliver a cationic lipid to the main channel while the second channel is used to deliver a nucleic acid. A droplet generation zone is provided in the main channel at the intersection of first and second carrier channels that contain a hydrophobic fluid. The cationic lipid, nucleic acid, and the hydrophobic fluid are then pumped through the device. In the droplet generation zone, shear force from the hydrophobic fluid pinches off droplets. The cationic lipid and nucleic acid are mixed in the generated droplets as the droplets move within a mixing region. A plurality of splitting channels may be coupled to the outlet of the device to produce smaller, monodisperse droplets having picoliter volumes.

REFERENCES:
patent: 2004/0068019 (2004-04-01), Higuchi et al.
patent: WO/96/32116 (1996-10-01), None
patent: WO 2004/071638 (2004-08-01), None
Tan et al Lab Chip, 2005, 5, 1178-1183.
Nisisako et al SICE, 2002, 957-959.
Atencia et al ,Nature, 2005, 437: 648-655.
Bertsch et al Proceedings of the IEEE 14th annual international conference on microelectro mechanical systems, MEMS 2001, Interlaken, Switzerland Jan. 21-25, 2001, pp. 507-510.
Fisher et al Presented on Sep. 26-30, 2004 published in 2005, pp. 647-649, at 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Malmo, Sweden.
Rousselle et al Journal of Biological Chemistry, 1996, 271, 726-735.
Nichols Lab Chip, 2006, 242, 6, 242-246, available online Dec. 8, 2005.
Song et al Lab Angew. Chem. Int. Ed. 2003, 42, No. 767-772.
TIce et al Langmuir 2003, 19, 9127-9133.
Bringer et al Philosophical transactions R. Soc. Lond. Series A, Mathematical, physical, and engineering sciences, (May 15, 2004) vol. 362, No. 1818, pp. 1087-1104.
Duffy, David C., et al., “Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane),” Anal. Chem. 1998, 70, 4974-4984.
Zelphati, O, et al., “Stable and Monodisperse Lipoplex Formulations for Gene Delivery,” Gene Therapy (1998) 5, 1272-1282.

Affiliated with

Also associated with

Rating

Microfluidic device for forming monodisperse lipoplexes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Microfluidic device for forming monodisperse lipoplexes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microfluidic device for forming monodisperse lipoplexes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4225705