Microfluidic assay system with dispersion monitoring

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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Details

C422S068100, C435S004000, C435S007100, C435S287100, C435S287900, C435S288200

Reexamination Certificate

active

07736891

ABSTRACT:
Disclosed is a microfluidic assay system and methods that apply flow injection analysis to permit dispersion monitoring. A solution containing a reagent that binds an analyte and a tracer is delivered via pressure-driven flow into the receiving end of the injection channel of the system of the invention. A sample fluid suspected of containing the analyte is delivered into the upstream end of the input channel under conditions permitting flow of the sample fluid toward the downstream end of the assay channel and permitting dispersion of the reagent into the sample fluid. The amount of tracer present in the fluid as it passes over the reference region and the capture region and the amount of binding between the analyte and the capture region are detected. The amount of binding detected between the analyte and the capture region is correlated to the amount of tracer detected in the reference region.

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