Microencapsulation with polymers

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435 1, 435178, 264 4, 935 54, 424422, 424424, 424425, C12N 500

Patent

active

048031682

ABSTRACT:
Disclosed is a process for damage-free encapsulation of a variety of core materials including viable cells. The core material is suspended in an aqueous solution of a polymeric material containing cationic groups such as an aminated glucopolysaccharide, e.g. chitosan. A temporary matrix is formed by gelling droplets of the suspension with a divalent or multivalent anion. The temporary matrix is permanently cross-linked with a polymeric material containing plural anionic groups, e.g., polyaspartic or polyglutamic acid, to form a semipermeable membrane. The interior of the microcapsule may be resolublized by subjecting the capsule to a solution of low molecular weight cations. The process produces microcapsules which are not sticky, do not clump and allow viable cell growth and proliferation.

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K. D. Vorlop et al. "Formation of Spherical Chitosan Viocatalysts by Ionotropic Gelation", Biotechnology Letters, vol. 3, No. 1, pp. 9-14 (1981).

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