Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-10-27
2000-10-17
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 600345, C12Q 168, C07H 2102, A61B 505
Patent
active
061329711
ABSTRACT:
A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.
REFERENCES:
patent: 4545382 (1985-10-01), Higgins et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4704353 (1987-11-01), Humphries et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4840893 (1989-06-01), Hill et al.
patent: 4883579 (1989-11-01), Humphries et al.
patent: 4908307 (1990-03-01), Rodland et al.
patent: 4945045 (1990-07-01), Forrest et al.
patent: 4963815 (1990-10-01), Hafeman
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5066372 (1991-11-01), Weetall
patent: 5108889 (1992-04-01), Smith
patent: 5112974 (1992-05-01), Barton
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5149630 (1992-09-01), Forrest et al.
patent: 5157032 (1992-10-01), Barton
patent: 5171853 (1992-12-01), Thorp et al.
patent: 5175082 (1992-12-01), Jeffreys
patent: 5194372 (1993-03-01), Nagai et al.
patent: 5272056 (1993-12-01), Burrows et al.
patent: 5278043 (1994-01-01), Bannwarth et al.
patent: 5312527 (1994-05-01), Mikkelsen et al.
patent: 5378628 (1995-01-01), Gratzel et al.
patent: 5405783 (1995-04-01), Pirrung et al.
patent: 5439829 (1995-08-01), Anderson et al.
patent: 5532129 (1996-07-01), Heller
patent: 5541113 (1996-07-01), Siddigi et al.
patent: 5545531 (1996-08-01), Rava et al.
patent: 5565322 (1996-10-01), Heller
patent: 5605662 (1997-02-01), Heller et al.
patent: 5632957 (1997-05-01), Heller et al.
patent: 5744305 (1998-04-01), Fodor et al.
patent: 5874219 (1999-02-01), Rava et al.
Evans et al., "A New Generation of DNA Chip Devices: Electronically controlled DNA hybridization on Semiconductors", 1995 AAAS Annual Meeting and Science Innovation Exposition: The 161st National Meeting of the American Association for the Advancement of, Feb. 1995.
Millan et al., "Sequence-Selective Biosensor for DNA Based on Electroactive Hybridization Indicators", Analytical Chemistry, vol. 65, pp. 2317-2323, Mar. 1993.
D. H. Johnston et al.; Electrochemical Measurement of the Solvent Accessibility of Nucleobases Using Electron Transfer between DNA and Metal Complexes, J. Am. Chem. Soc. 117: 8933-8938 (1995).
K. M. Millan et al.; Sequence-Selective Biosensor for DNA Based on Electroactive Hybridization Indicators, Anal. Chem. 65:2317-2323 (1983).
W. Bains; The Chip of the 90s, Chem. in Britian 122-125 (Feb. 1995).
T. J. Meade et al.; Electron Transfer through DNA: Site-Specific Modification of Duplex DNA with Ruthenium Donors and Acceptors, Angew. Chem. Int. Ed. Engl. 34 No. 3:352-354 (1995).
S. P. A. Fodor et al.; Multiplexed biochemical assays with biological chips Product Review 364:555-556 (1993).
S. P. A. Fodor et al.; Light-Directed, Spatially Addressable Parallel Chemical Synthesis, Science 251: 767-773 (1991).
Z. Du et al.; Automated Fluorescent DNA Sequencing of Polymerase Chain Reaction Products, Methods in Enzymology 218: 104-121 (1993).
J. M. Hall et al.; An Electochemical Method for Detection of Nucleic Acid Hybridisation, Biochem. and Molecular Bio. Int'l. 32: No. 1, 21-28 (1994).
D. Noble; DNA Sequencing on a Chip, Anal. Chem. 67, No. 5:201-204 (1995).
Y. Jenkins et al.; A Sequence-Specific Molecular Light Switch: Tetherin of an Oligonucleotide to a Dipyridophenazine Complex of Ruthenium(II), J. Am. Chem. Soc. 114:8736-8738 (1992).
K. M. Millan et al.; Voltammetric DNA Biosensor for Cystic Fibrosis Based on a Modified Carbon Paste Electrode, Anal. Chem. 66: 2943-2948 (1994).
M. T. Carter et al.; Voltammetric Studies of the Interaction of Metal Chelates with DNA. 2. Tris-Chelated Complexes of Colbalt (III) and Iron (II) with 1, 10-Phenanthroline and 2,2'-Bipyridine, J. Am. Chem. Soc. 111:8901-8911 (1989).
S. A. Strobel et al.; Minor Groove Recognition of the Conserved G.U Pair at the Tetrahymena Ribozyme Reaction Site, Science 267:675-679 (1995).
T. Ried et al.; Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy, Proc. Natl. Acad. Sci. USA 89:1388-1392 (1992).
R. Tizard et al.; Imaging of DNA sequences with chemiluminescence, Proc. Natl. Acad. Sci. USA 87:4514-4518 (1990).
A. Lishanski et al.; Mutation detection by mismatch binding protein, MutS, in amplified DNA: Application to the cystic fibrosis gene, Proc. Natl. Acad. Sci. USA 91:2674-2678 (1994).
C. J. Murphy et al; Fast photoinduced electron transfer through DNA intercalation, Proc. Natl. Acad. Sci. USA 91:5315-5319 (1994).
S. A. Strobel et al; Site-Specific Cleavage of a Yeast Chromosome by Oligonucleotide-Directed Triple-Helix Formation, Science 249:73-75 (1990).
C. J. Murphy et al; Long-Range Photoinduced Electron Transfer Through a DNA Helix, Science 262:1025-1029 (1993).
D. H. Johnston et al; Trans-Dioxorhenium(V)-Mediated Electrocatalytic Oxidation of DNA at Indium Tin-Oxide Electrodes: Voltammetric Detection of DNA Cleavage in Solution, Imorg. Chem. 33:6388-6390 (1994).
M. Maeder et al; Nonlinear Least-Squares Fitting of Multivariate Absorption Data, Anal. Chem. 62:2220-2224 (1990).
M. Rudolph et al; A Simulator for Cyclic Voltammetric Responses, Analytical Chemistry 66:589-600 (1994).
J. Ostteryoung; Voltammetry for the Future, Acc. Chem. Res. 26 No. 3: 77-83 (1993).
M. A. Tracey et al; Dynamics of Rigid and Semirigid Rodlike Polymers, Annu. Rev. Phys. Chem. 43:525-557 (1992).
A. M. Pyle et al; Mixed-Ligand Complexes of Ruthenium(II): Factors Governing Binding to DNA, J. Am. Chem. Soc. 111:3051-3058 (1989).
O. S. Fedorova et al; Application of Tris(2,2'-bipyridyl) ruthenium(III) for the Investigation of DNA Spatial Structure by a Chemical Modification Method, Journal of Inorganic Biochemistry 34:149-155 (1988).
S. Satyanarayana, et al; Neither .sub..DELTA. -nor .sub..LAMBDA. -Tris(phenanthroline)ruthenium(II) Binds to DNA by Classical Intercalation; Biochemistry 31 No. 39:9319-9324 (1992).
J. A. Saleeba et al; [19]Chemical Cleavage of Mistmatch of Detect Mutations, Methods in Enzymology 217: 286-295 (1993).
S. Steeken et al; One-Electron-Reduction Potentials of Pyrimidine Bases, Nucleosides, and Nucleotides in Aqueous Solution. Consquences for DNA Redox Chemistry, J. Am. Chem. Soc. 114: 4701-4709 (1992).
K.R. Khrapko et al; Hybridization of DNA with oligonucleotides immobilized in gel: convient method for detection of single base changes, Mol. Biol. 25(3): 718 (1991).
L. J. Mather III; Inhibition of T7 RNA Polymerase Intiation by Triple-Helical DNA Complexes: A Model for Artificial Gene Repression, Biochemistry 31 No. 33; 7587-7594 (1992).
Adams et al.; editors The Biochemistry of Nucleic Acids, Chapman & Hall, New York, pp 519-524 (1992).
Johnston Dean H.
Kim Jin-heung
Loomis Carson R.
Napier Mary E.
Sistare Mark F.
Chakrabarti Arun
Fredman Jeffrey
The University of North Carolina at Chapel Hill
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