Microdialysis unit for molecular weight separation

Details Microdialysis unit for molecular weight separation Microdialysis unit for molecular weight separation

1997-05-09

1999-09-21

Kim, John

Liquid purification or separation

Casing divided by membrane into sections having inlet

Each section having inlet

210259, 2103216, 21032187, B01D 6128, B01D 6302

Patent

active

059549599

ABSTRACT:
The present invention relates generally to an apparatus and method for separating high molecular weight molecules from low molecular weight molecules. More specifically, the invention relates to the use of microdialysis for removal of the salt (low molecular weight molecules) from a nucleotide sample (high molecular weight molecules) for ESI-MS analysis. The dialysis or separation performance of the present invention is improved by (1) increasing dialysis temperature thereby increasing desalting efficiency and improving spectrum quality; (2) adding piperidine and imidazole to the dialysis buffer solution and reducing charge states and further increasing detection sensitivity for DNA; (3) using low concentrations (0-2.5 mM NH4OAc) of dialysis buffer and shifting the DNA negative ions to higher charge states, producing a nearly 10-fold increase in detection sensitivity and a slightly decreased desalting efficiency, or (4) any combination of (1), (2), and (3).

REFERENCES:
patent: 3442389 (1969-05-01), Mendelson
patent: 4491011 (1985-01-01), Nordmeyer et al.
patent: 4781889 (1988-11-01), Fukasawa et al.
patent: 4794088 (1988-12-01), Miyaki et al.
patent: 4804628 (1989-02-01), Cracauer et al.
patent: 4921952 (1990-05-01), Longmire et al.
patent: 5078135 (1992-01-01), Caprioli
patent: 5202023 (1993-04-01), Trimmer et al.
patent: 5449064 (1995-09-01), Hogan et al.
patent: 5492838 (1996-02-01), Pawliszyn
patent: 5572023 (1996-11-01), Caprioli
patent: 5706806 (1998-01-01), Kissinger

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