Coating processes – Optical element produced – Transparent base
Reexamination Certificate
2006-11-23
2011-11-15
Warden, Jill (Department: 1773)
Coating processes
Optical element produced
Transparent base
C427S162000, C422S547000, C422S552000, C422S553000
Reexamination Certificate
active
08057852
ABSTRACT:
Glass as a substrate for fluorescence-based assays is expensive. Disclosed is a poly(cyclic olefin) alternative to glass that is of comparable sensitivity. More specifically, disclosed is a method of making a microdevice for the immobilization of biomolecules for the purpose of carrying out a fluorescence-based assay, said method comprising providing a substrate comprising a poly(cyclic olefin); protecting the substrate from ultraviolet (UV) light; and subjecting a surface of said UV-protected substrate to ozone oxidation to activate said surface; wherein a content of intrinsic fluorophores on the surface of the substrate remains substantially unchanged after the ozone oxidation. Also disclosed is a microdevice for the immobilization of biomolecules for the purpose of carrying out a fluorescence-based assay, said microdevice comprising a body comprising a poly(cyclic olefin), said body having an ozone-activated surface substantially free of intrinsic fluorophores; wherein said activated surface is substantially free of intrinsic fluorescence during a fluorescence-based assay of said biomolecules bound to said activated surface.
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Diaz-Quijada Gerardo A.
Veres Teodor
(Marks & Clerk)
Handy Dwayne K
Mitchell Richard J.
National Research Council of Canada
Warden Jill
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